We narrowed to 159,557 results for: addgene

1. pLKO mouse shRNA 2 raptor

   Plasmid

   #21340

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 13. ():.

   Insert

   raptor shRNA 2 (Rptor Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pX330-NEAT1m_v1

   Plasmid

   #97083

   Purpose

   Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1m_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

   Plasmid

   #170182

   Purpose

   Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   mCherry
   

   Use

   Tags

   3XFlag and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL-1

   Plasmid

   #180422

   Purpose

   Kan resistant plasmid derived from pBTK402, a broad-host-range plasmid previously shown to replicate in multiple species of bee gut bacteria. It encodes a low level RFP promoter.

   Depositor

   Nancy Moran

   Article

   Li et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Red Chromprotein gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC_C_AOX

   Plasmid

   #111661

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   AOX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH-roGFP2-GPXC71S

   Plasmid

   #191698

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a control roGFP2 fusion not responsive to peroxides used alongside plasmids 191680 and 191677

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   redox(ro)GFP2-GPXC71S
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Peroxidatic Cysteine 71 replaced with Serine

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX335-NEAT1_IS_v1

   Plasmid

   #97085

   Purpose

   Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1_IS_v1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CBh-DIO-CB2-EGFP

   Plasmid

   #91946

   Purpose

   Expression of mouse CB2 cannabinoid receptors and eGFP in the presence of Cre

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Inserts

   EGFP
   type-2 cannabinoid receptor
   

   Use

   AAV

   Tags

   FLAG and None

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRG389-PC2

    Plasmid

    #99087

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone convertase 2 (PC2)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    prohormone convertase 2 (PC2)
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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