We narrowed to 10,522 results for: SEC

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VP16-ER beta (long)

Plasmid

#11352

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta long (ESR2 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP4

Plasmid

#129507

Purpose

Expresses AvicFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP4

Expression

Bacterial

Mutation

One of two variants of AvicFP4 tested with essent…

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2-Clover

Plasmid

#191008

Purpose

Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Clover

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pKDR-Lenti-CBh.6-SOD1

Plasmid

#188460

Purpose

For knockdown-replacement of SOD1 gene (SOD1 KDR lentiviral transfer plasmid, Fig 4)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

SOD1 (SOD1 Human)

Use

Lentiviral

Promoter

CBh

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab1(Y628F/Y660F)

Plasmid

#188658

Purpose

FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 1 (Gab1 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y628F and Y660F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pFB-GST-IRP2

Plasmid

#226621

Purpose

Expresses human IRP2 in insect cells

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Insert

IRP2 (IREB2 Human)

Tags

GST

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGN

Plasmid

#236512

Purpose

For expression of Cingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAWG-dCas9-VPR

Plasmid

#63802

Purpose

SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

SP-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Insect

Promoter

act5c

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pOmniBac zz TEV YBBR TRF1

Plasmid

#185445

Purpose

Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Insert

TRF1 (TERF1 Human)

Tags

YBBR and ZZ

Expression

Insect

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe DN-FOXO1-HA neo

Plasmid

#45814

Depositor

Kevin Janes

Article

Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

Insert

FOXO1 (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

aa1-255

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pCLHCX-mPM20D1-flag

Plasmid

#84566

Purpose

mouse PM20D1, C-term flag and his tags, in mammalian retroviral expression plasmid. Amp/Hygro selection

Depositor

Bruce Spiegelman

Article

Long et al Cell. 2016 Jul 14;166(2):424-35. doi: 10

Insert

peptidase M20 domain containing 1 (Pm20d1 Mouse)

Use

Retroviral

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-3UAG

Plasmid

#82501

Purpose

Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescence Protein with 3 UAG codons

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3, …

Promoter

AraBAD

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N116C-G193C

Plasmid

#162580

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N116C-G193C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Mutation

Asparagine 116 was mutated to Cysteine and Glycin…

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC6

Plasmid

#224346

Purpose

Expresses human KDAC6 (HDAC6) in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RH4-bio

Plasmid

#47779

Purpose

Expresses enzymatically monobiotinylated partial RH4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RH4 (partial)

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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