We narrowed to 6,717 results for: SIM;

1. pET41a-MED18

   Plasmid

   #15433

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

   Insert

   MED18 (MED18 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pT2EFCRET(#324)

   Plasmid

   #184059

   Purpose

   cyclofen-inducible CRE activation in zebrafish transient transgenic

   Depositor

   Sophie Vriz

   Article

   Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

   Insert

   CRE-ERT2
   

   Use

   Zebrafish transient transgenesis

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Xl-EF1a

   Available since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)

   Plasmid

   #51369

   Depositor

   Narry Kim

   Article

   Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   3' pocket mutant (Y926F, R927A)

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

4. HOXB4 (human) HA-TAT-tag pET

   Plasmid

   #8526

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   replace T7 and HIS tags of pET with an HA tag;

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-FLAG-Dicer-5'-pocket mutant

   Plasmid

   #51368

   Depositor

   Narry Kim

   Article

   Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

   Insert

   Dicer (DICER1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   5' pocket mutant (R778A, R780A, R811A, H982A…

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

6. HBM-mVEGF-C-HisTag-pENTR

   Plasmid

   #64066

   Purpose

   Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

   Insert

   vascular endothelial growth factor C (Vegfc Mouse)
   

   Use

   Tags

   His-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN_TGmiR_Gb2-K

   Plasmid

   #25766

   Purpose

   Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Gb2 miR-shRNA (Gnb2 Mouse)
   

   Use

   Entry vector

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMini-CAAGs::RFP-DR48

   Plasmid

   #132640

   Purpose

   Fluorescent reporter to quantify elicitation of the strand annealing mediated repair pathway

   Depositor

   Stephen Ekker

   Article

   Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

   Insert

   mRFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains a 92bp insertion interrupting the open …

   Promoter

   Chicken B-actin

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS664-pMCSG7-AcrIIC5Smu

   Plasmid

   #113441

   Purpose

   Expresses a 6X-His tagged type II-C anti-CRISPR protein from S. muelleri in bacterial cells

   Depositor

   Erik Sontheimer

   Article

   Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

   Insert

   Type II-C anti-CRISPR with N-terminal 6xHis and TEV site
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   lacI

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET41a-MED22

    Plasmid

    #15432

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED22 (MED22 Human)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pGAL4-DBD-TBX1wt-IDR

    Plasmid

    #145254

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1wt-IDR (TBX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R725-X09-635: His6-tev-Hs.SPRED1(13-125)

    Plasmid

    #159581

    Purpose

    Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Insert

    His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP AH2 tensin

    Plasmid

    #50514

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.

    Insert

    Tensin (TNS1 Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AA659--761, including actin homology 2 domain of …

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. P63-sp64 Poly A

    Plasmid

    #59435

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 63
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

17. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-BE3-PGK-Puro

    Plasmid

    #110842

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET32b-MED19

    Plasmid

    #15438

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED19 (Med19 Mouse)
    

    Use

    Tags

    His and Trx

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

    Plasmid

    #113437

    Purpose

    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    Type II-C anti-CRISPR
    

    Use

    CRISPR

    Tags

    FLAG/NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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