We narrowed to 24,925 results for: crispr
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Plasmid#159903PurposeMutagenesis of Oprk1DepositorInsertOprk1 (Oprk1 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AcrIIA2
Plasmid#101042PurposeAnti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.DepositorInsertAcrIIA2
TagsGSTExpressionBacterialAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pV1326
Plasmid#111435PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEC1.2/EC1.1_spCas9_TagRFP
Plasmid#161934PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - TagRFP seed selectionDepositorTypeEmpty backboneUseCRISPRAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA351-Cpf1
Plasmid#192361PurposeCRISPR-based vector for genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas12a, gRNA scaffold
UseCRISPRAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralExpressionMammalianMutationH231LAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJZC33
Plasmid#62330PurposesgRNA + 2x MS2 with MCP-VP64 effector for mammalian cellsDepositorInsertssgRNA + 2x MS2 binding module
MCP-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVT36b
Plasmid#184437PurposeCRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCas9–mKate2ps–CgRNA
Plasmid#64955PurposeControl gRNA (GTCAAGGCACTCTTGCCTA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9–mKate2ps
ExpressionMammalianAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEG302 22aa SunTag VP64 g4+g17
Plasmid#120252PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAsDepositorInsertg17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceMarch 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC020 - LseCas13a from Listeria seeligeri
Plasmid#91910PurposeExpresses LseCas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLseCas13a and guide expression cassette
ExpressionBacterialAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZ8-P_dCas9
Plasmid#74063PurposepZ8-1 plasmid carrying dcas9 driven by the propionate-inducible prpD2 promoter (PprpD2), KanRDepositorPublicationInsertdcas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPropionate inducible promoter (prp)Available sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G5_Array
Plasmid#86620PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via hdr using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCD185
Plasmid#113317PurposedCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCMV-NLS-BvCas12b-NLS-3xHA
Tags3XHAExpressionMammalianAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRExpressionBacterialAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RAD21_1
Plasmid#64057PurposeExpresses gRNA against human RAD21 along with Cas9 with 2A GFPDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA
UseCRISPRAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
HPRT2b-pGEM
Plasmid#69539PurposegRNA targeting human HPRT geneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHPRT gRNA2
ExpressionMammalianAvailable sinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dLwaCas13a-EGFP-NLS-3xFlag
Plasmid#132407Purposeoverexpression in human cellsDepositorInsertdLwaCas13b
UseLentiviralTagsflagExpressionMammalianMutationR474A; H479A; R1046A; R1051AAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by