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We narrowed to 31,261 results for: son

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Showing: 5041 - 5060 of 31261 results

  3. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available Since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  4. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  6. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Promoter
    Tef1
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

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  7. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196078-plasmid-map-sequence-id-388989

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  10. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139093
    Purpose
    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9-NG-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139093-plasmid-map-sequence-id-273890

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  12. pBJ3

    Plasmid
    #167132
    Purpose
    A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter
    Depositor
    Akira Mine
    Article
    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d
    Insert
    luxCDABE
    Use
    Luciferase
    Expression
    Bacterial
    Promoter
    Frr promoter
    Available Since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167132-plasmid-map-sequence-id-330441

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  13. PB-CA sfGFP

    Plasmid
    #159094
    Purpose
    PB-CA sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Piggybac
    Expression
    Mammalian
    Available Since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159094-plasmid-map-sequence-id-316224

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  15. pDL1

    Plasmid
    #182263
    Purpose
    Gibson cloning vector for synthesis of single and double stranded RNA
    Depositor
    Daniel Lobo
    Article
    Wolff et al STAR Protoc. 2022 Jun 14;3(3):101458. do
    Type
    Empty backbone
    Use
    Gibson cloning vector for synthesis of single and…
    Promoter
    T3 and SP6 for sense and antisense riboprobe synt…
    Available Since
    June 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    182263-plasmid-map-sequence-id-360127

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  16. AAV-CAG-flex-lck-smV5-4x6T

    Plasmid
    #196419
    Purpose
    Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassette
    Depositor
    Stanley Thomas Carmichael
    Article
    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:
    Insert
    Lck-smV5
    Use
    AAV and Cre/Lox; Astrocyte-selective
    Tags
    Lck
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    196419-plasmid-map-sequence-id-385571

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  17. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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Showing: 5041 - 5060 of 31261 results