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p21-M694I-MEFV

Plasmid

#134710

Purpose

Lentiviral expression of human MEFV M694I after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.M694I

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-S208C/M694V-MEFV

Plasmid

#134709

Purpose

Lentiviral expression of human MEFV S208C/M694V after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.S208C/M694V

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p21-M694V-MEFV

Plasmid

#134703

Purpose

Lentiviral expression of human MEFV M694V after doxycycline administration

Depositor

Thomas Henry

Article

Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Mutation

p.M694V

Promoter

TRE2 (Dox-inducible)

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Hgs-g1)-PGKpuroBFP-W

Plasmid

#105032

Purpose

Lentiviral gRNA plasmid targeting mouse Hgs , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

gRNA targeting Hgs (Hgs Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6AP III (C41A, C46A, C840A)

Plasmid

#119318

Purpose

lentiviral vector for expression of HA-Tagged E6-AP III (C41A, C46A, C840A) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP III (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C41A, C46A, C840A

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-dTPR

Plasmid

#114048

Purpose

Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-dTPR (TTK Human)

Expression

Mammalian

Mutation

delta TPR (deletion of TPR domain 63-191)

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d60

Plasmid

#114046

Purpose

Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d60 (TTK Human)

Expression

Mammalian

Mutation

delta 60 (deletion of NTE domain)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCOG40-HRS (257-276) Hs

Plasmid

#83236

Purpose

pGST-HRS (257-276) Hs, Expresses the GST-HRS in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

HRS (257-276) Hs (HGS Human)

Expression

Bacterial

Mutation

258-277

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001487098)

Plasmid

#78031

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001148014)

Plasmid

#78028

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001146579)

Plasmid

#78029

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CKM gRNA (BRDN0001148653)

Plasmid

#78030

Purpose

3rd generation lentiviral gRNA plasmid targeting human CKM

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CKM (CKM Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001146806)

Plasmid

#78001

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001145144)

Plasmid

#78002

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001144933)

Plasmid

#78003

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FN3KRP gRNA (BRDN0001487148)

Plasmid

#78004

Purpose

3rd generation lentiviral gRNA plasmid targeting human FN3KRP

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FN3KRP (FN3KRP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001146460)

Plasmid

#75986

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001148530)

Plasmid

#75984

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CAMK1G gRNA (BRDN0001147776)

Plasmid

#75985

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK1G

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK1G (CAMK1G Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5-L518A/Y619E-FRAG

Plasmid

#68874

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa, L518A/Y619E

Promoter

tac

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only