We narrowed to 10,795 results for: HA

1. pAG415Gal_Gcn4 Aro2+GFP

   Plasmid

   #233001

   Purpose

   Galactose iduced expression of Gcn4 Aro2+GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Aro2+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N116F, S117E, E119N, P129Q, T132Y

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 9acidAGFP

   Plasmid

   #233009

   Purpose

   Galactose iduced expression of Gcn4 9acidAGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 9acidA
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 Aro3+GFP

   Plasmid

   #233002

   Purpose

   Galactose iduced expression of Gcn4 Aro3+GFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Aro3+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103Y, N112T, L113Y, S117F, K140R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-BioID2-HA3-KanMX6

   Plasmid

   #227976

   Purpose

   To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   BioID2
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-BirA-HA3-KanMX6

   Plasmid

   #227974

   Purpose

   To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   BirA
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. β2AR-PPI-CALPon1.0 (TEVp)

   Plasmid

   #213407

   Purpose

   Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 2)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   HA-arrestin-TEVp
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-MBP-yATOM_WDR5

   Plasmid

   #209706

   Purpose

   Mammalian expression of y-ATOM biosensor for WDR5

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_WDR5
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MBP-yATOM_SH2

   Plasmid

   #209708

   Purpose

   Mammalian expression of y-ATOM biosensor for SH2

   Depositor

   Stewart Loh

   Article

   Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

   Insert

   MBP-yATOM_SH2
   

   Use

   Tags

   MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PRLSS-yATOM_SH2-KDEL

    Plasmid

    #209714

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-yATOM_SH2-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-MBP-yATOM_WDR5 (R80A)

    Plasmid

    #209713

    Purpose

    Mammalian expression of y-ATOM biosensor for WDR5 with binding null R80A mutation

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    MBP-yATOM_WDR5 (R80A)
    

    Use

    Tags

    MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

    Plasmid

    #209718

    Purpose

    Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-NbGFP_miRFP670nano3-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-PRLSS-Clover-KDEL

    Plasmid

    #209720

    Purpose

    Mammalian expression of clover localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-Clover-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Cox4-yATOM_SH2

    Plasmid

    #209715

    Purpose

    Mammalian expression of y-ATOM biosensor for SH2 localized to mitochondria

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    Cox4-yATOM_SH2
    

    Use

    Tags

    Cos4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. KBB382

    Plasmid

    #185085

    Purpose

    HA-tagged POM152 with glycosylation sites mutated

    Depositor

    Ken Belanger

    Article

    Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

    Insert

    POM152
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

16. pT22i5uasH3En2SE4P3Che(#1212)

    Plasmid

    #184098

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Insert

    3HA-chkEn2(C>S)-ERT2-5'fP2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    3xHA

    Expression

    Mutation

    chkEn2: C175S; 5'fP2A: GSG-P2A; ERT2: G400V,…

    Promoter

    5xUAS

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColE1-AlphaOmega

    Plasmid

    #165867

    Purpose

    Terminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis(TEV)-yDrp1-StrepII

    Plasmid

    #174427

    Purpose

    Expresses S. pombe Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBlastR-GMRWhite

    Plasmid

    #165885

    Purpose

    Blasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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