We narrowed to 11,787 results for: nsf

1. pAAV-hSynI-EGFP-WPRE-hGH poly(A) (Alex_02)

   Plasmid

   #71651

   Purpose

   AAV expression of EGFP in human and mouse neurons

   Depositor

   Mirjana Maletić-Savatić

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-FRT-MCS-WPRE-SV40 - GCH27

   Plasmid

   #230828

   Purpose

   AAV cloning vector for Flpo-dependent expression under the TRE promoter.

   Depositor

   Kimberly Ritola

   Type

   Empty backbone

   Use

   AAV; Cloning vector, flp/frt

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-hSyn-PiGM-Iq(535/81, eGFP)

   Plasmid

   #221612

   Purpose

   AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).

   Depositor

   John Lin

   Insert

   PiGM-Iq (535/81,eGFP)
   

   Use

   AAV

   Mutation

   RGS2 1-53 truncation

   Promoter

   Human Synapsin

   Available Since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

   Plasmid

   #223504

   Purpose

   Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

   Depositor

   James Frank

   Insert

   dead-mutant SNAP-tag and EGFP
   

   Expression

   Mammalian

   Mutation

   SNAP-tag C145A

   Promoter

   hSyn

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-GFAPp-PiGM-Iq(eGFP)

   Plasmid

   #221606

   Purpose

   A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. GFAP promoter for astrocyte-selective expression.

   Depositor

   John Lin

   Insert

   PiGM-Iq (eGFP)
   

   Use

   AAV

   Mutation

   RGS2 1-53 truncation

   Promoter

   GFAP promoter

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-Hipk1

   Plasmid

   #221508

   Purpose

   Retrovirus-mediated gene transfer into mammalian cells to overexpress Hipk1

   Depositor

   Alessandro Ori

   Insert

   Hipk1 (Hipk1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

   Plasmid

   #219689

   Purpose

   Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).

   Depositor

   Jennifer Nemhauser

   Insert

   T2PsB (mtagBFP2, mCherry, NeonGreen)
   

   Expression

   Plant

   Available Since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-SYN-eGFP-oPRE

   Plasmid

   #218681

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

   Depositor

   Bart Nieuwenhuis

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRS_8-somGtACR2-eGFP

   Plasmid

   #192590

   Purpose

   AAV-vector for optogenetic silencing of noradrenergic neurons

   Depositor

   Simon Wiegert

   Insert

   somGtACR2
   

   Use

   AAV

   Tags

   EGFP - soma-targeting motif from Kv2.1 channel

   Expression

   Mammalian

   Promoter

   PRSX8

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid

    #183704

    Purpose

    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit

    Depositor

    Jonathan Ploski

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    CaMKIIa

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pISV-DsRed1-nopP

    Plasmid

    #171025

    Purpose

    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.

    Depositor

    Christian Staehelin

    Insert

    nopP
    

    Expression

    Plant

    Promoter

    2 X EN 35S

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. FLJ20628

    Plasmid

    #25481

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    FLJ20628 (TRMT61B Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pSBBi-GB CaXMT1

    Plasmid

    #139168

    Purpose

    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.

    Depositor

    Neal Devaraj

    Insert

    7-methylxanthosine synthase 1 [Coffea arabica]
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1α

    Availability

    Academic Institutions and Nonprofits only

    

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14. p4X-UASTattB

    Plasmid

    #189858

    Purpose

    Fly cloning and transformation

    Depositor

    Jung Hwan Kim

    Article

    Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-phSyn-flex-SypEGFP

    Plasmid

    #153203

    Purpose

    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

    Depositor

    Lynette Lim

    Insert

    synaptophysin-EGFP
    

    Use

    AAV

    Promoter

    rat synapsin

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.hChR2(H134R)-mCherry.WPRE.SV40

    Plasmid

    #100054

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry driven by CAG promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV1, AAV2, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid

    #135391

    Purpose

    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

    Depositor

    Fan Wang

    Insert

    TeLC-P2A-EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid

    #202036

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM3Dq
    

    Use

    AAV

    Tags

    dTomato

    Promoter

    CAG

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-CAG-hM4Di-P2A-dTomato

    Plasmid

    #202037

    Purpose

    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM4Di-2A-dTomato

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Insert

    hM4Di
    

    Use

    AAV

    Tags

    dTomato

    Promoter

    CAG

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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