We narrowed to 14,476 results for: nts

1. pEPOR2KN0039

   Plasmid

   #117606

   Purpose

   Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   [35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
   

   Expression

   Plant

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-his-3C_Clu3B6p

   Plasmid

   #100093

   Purpose

   expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

   Insert

   14-3-3 sigma human (SFN Human)
   

   Tags

   HSPB6 peptide and His6

   Expression

   Bacterial

   Mutation

   75-77 residues changed to AAA to facilitate cryst…

   Promoter

   T7

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB105-mTalin

   Plasmid

   #100584

   Purpose

   Binary vector for expression of Citrine-fused mouse Talin (Citrine-mTalin) to visualize actin filaments in plants

   Depositor

   Yutaka Kodama

   Article

   Kimura et al PeerJ. 2016 Sep 28;4:e2513. eCollection

   Insert

   Citrine-mTalin (Tln1 Mouse)
   

   Expression

   Plant

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ290-D570A

   Plasmid

   #129673

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b
   

   Use

   CRISPR; Gateway compatible aaccas12b-d570a entry …

   Expression

   Plant

   Mutation

   D570A; AacCas12b is rice codon optimized

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

   Plasmid

   #68278

   Purpose

   SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

   Depositor

   Serge Pelet

   Article

   Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

   Insert

   Ste7DS NLS mCitrine
   

   Tags

   mCitrine

   Expression

   Yeast

   Promoter

   pRPS2

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cdc42 T17N pCS2

   Plasmid

   #16670

   Depositor

   De-Li Shi

   Article

   Djiane et al Development. 2000 Jul . 127(14):3091-100

   Insert

   Cdc42 T17N (CDC42 Human)
   

   Use

   Vertebrate

   Mutation

   T17N. Dominant negative.

   Available Since

   April 28, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pYPQ293 (BhCas12b_v4)

   Plasmid

   #136383

   Purpose

   Gateway entry clone for BhCas12b_v4

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BhCas12b_v4
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   K846R/S893R/E837G

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL712

   Plasmid

   #140382

   Purpose

   Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-otrO-3WJdB-T
   

   Use

   Synthetic Biology

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTX201

   Plasmid

   #89265

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A3601

    Plasmid

    #91051

    Purpose

    Module A, Promoter: 35S, Gene: dAtCas9-VP64, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    dAtCas9-VP64
    

    Use

    CRISPR

    Promoter

    35S

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1 human APC-2,3 (1362–1540)

    Plasmid

    #132631

    Purpose

    expresses human APC-2,3 (1362–1540) in E. coli

    Depositor

    Wenqing Xu

    Article

    Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

    Insert

    APC-2,3 (1362–1540)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH71

    Plasmid

    #128214

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUPI AoT5Cyp

    Plasmid

    #80280

    Purpose

    Expresses Aotus TRIM5 CypA fusion

    Depositor

    Jeremy Luban

    Article

    Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

    Insert

    TRIM5 CypA fusion
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5_tAKARζ

    Plasmid

    #119918

    Purpose

    Mammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARζ
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEPOR2KN0079

    Plasmid

    #117646

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_ENTH/ANT {AT1G14910}(pEPOR1CB0100)and sgRNA_ENTH/ANT {AT2G01600}(pEPOR1CB0111)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-1459-adcy4-Luc

    Plasmid

    #28184

    Depositor

    Bernard Schimmer

    Article

    Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

    Insert

    Adcy4 promoter (Adcy4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. CP1300-CBEmax

    Plasmid

    #119805

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    rApobec1-nSpCas9 (CP1300) -UGI-UGI
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT mir-1-1 reporter cel-mir-40

    Plasmid

    #46739

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    cel-mir-40
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-SZT2 1-1021

    Plasmid

    #87037

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 1-1021

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Mutation

    K810A, K1003A, R1060A

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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