We narrowed to 10,392 results for: plasmids 101

1. pCXLE-hOCT3/4

   Plasmid

   #27076

   Purpose

   Non-integrating (episomal) expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   OCT3/4 (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCathepsin S

   Plasmid

   #11251

   Purpose

   Mammalian expression of human Cathepsin S

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Insert

   cathepsin S (CTSS Human)
   

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pIVM_02

   Plasmid

   #204500

   Purpose

   Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

   Depositor

   Alessio Ciulli

   Article

   Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

   Insert

   amino acids 54-213 VHL (VHL Human)
   

   Tags

   6xHis, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   0

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScI_dCas9-CDA_J23119-sgRNA

   Plasmid

   #108550

   Purpose

   Bacterial Target-AID vector with sgRNA

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Inserts

   dCas9-PmCDA1
   Streptococcus pyogenes sgRNA
   

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   J23119 and lambda OR

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-MLS-KillerRed

   Plasmid

   #115517

   Purpose

   Middle entry plasmid containing membrane bound KillerRed fluorophore

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9 fused to nanobody (anti-beta catenin)

   Plasmid

   #186420

   Purpose

   Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

   Depositor

   Stephen Elledge

   Article

   Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

   Insert

   dCas9-nanobody fusion (anti-beta catenin)
   

   Use

   CRISPR

   Tags

   6His

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKHR8

   Plasmid

   #74625

   Purpose

   a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

   Depositor

   David Grunwald

   Article

   Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

   Inserts

   FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
   cmlc2::mCherry
   

   Use

   Cre/Lox; Zebrafish expression

   Promoter

   alpha-crystallin and cmlc2

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHOHO2_Hsp82viiA

   Plasmid

   #61708

   Purpose

   Yeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.

   Depositor

   Dan Bolon

   Article

   Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

   Insert

   Hsp82viiA (HSP82 Budding Yeast)
   

   Use

   Yeast integration vector targeting inserts to the…

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Heteromeric coiled coil inserted after amino acid…

   Promoter

   TEF1

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

   Plasmid

   #141094

   Purpose

   Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

   Depositor

   Carolyn McBride

   Article

   Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

   Insert

   bruchpilot
   

   Use

   CRISPR

   Expression

   Insect

   Mutation

   coding sequence downstream of CRISPR cut site, sy…

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFD115

    Plasmid

    #124768

    Purpose

    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

    Depositor

    David Bikard

    Article

    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

    Inserts

    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    

    Use

    CRISPR

    Promoter

    Ptet

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Cas13d-T2A-Citrine

    Plasmid

    #224565

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    Cas13d-T2A-Citrine
    

    Use

    CRISPR

    Tags

    T2A-Citrine

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

    Plasmid

    #164800

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA1
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

    Plasmid

    #164801

    Purpose

    For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    ERH CRISPRi gRNA2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTwist Amp_2xFlag-dSERT-HDR-donor-HDR

    Plasmid

    #221835

    Purpose

    Donor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frame

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    2xFLAG with dSERT homology arms
    

    Use

    CRISPR

    Expression

    Insect and Mammalian

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-22b(+) pCopA RBS30 sfgfp

    Plasmid

    #226371

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Tags

    6x HisTag

    Expression

    Bacterial

    Promoter

    copA promoter (pCopA) and RBS30 regulation

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

    Plasmid

    #176575

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q W330Y

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC_h7SK M15 (UUA)

    Plasmid

    #154785

    Purpose

    plasmid with single copy of Mma PylT M15 mutant (UUA anticodon)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    Mma PylT
    

    Expression

    Mammalian

    Mutation

    UUA anticodon and G10A, G14U, G16U, C20U, C52A

    Promoter

    h7SK

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCXLE-hOCT3/4-shp53-F

    Plasmid

    #27077

    Purpose

    Integration-free (episomal) expression of human OCT3/4 and shRNA against p53

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    OCT3/4 (POU5F1 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DIO-ATLASsnFlp

    Plasmid

    #232352

    Purpose

    Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons that express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells.

    Depositor

    Don Arnold

    Article

    Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

    Insert

    ATLASsnFlp
    

    Use

    AAV

    Tags

    ALFA-Tag

    Promoter

    hSyn

    Available Since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. hCathepsin B

    Plasmid

    #11249

    Purpose

    Mammalian expression of human cathepsin B

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Insert

    cathepsin B (CTSB Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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