We narrowed to 12,952 results for: sequence
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Plasmid#202164PurposeLevel 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.DepositorInsertfcpA terminator
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable SinceAug. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF1a-T2-CeNLuc
Plasmid#135948PurposeExpresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertCeNLuc LumiFluor
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
GAL4-mNFAT1(2-144)
Plasmid#11108PurposeExpresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTagsGAL4-DBDExpressionMammalianMutationContains NFAT1 amino acids 2-144; L78P and P140Q …PromoterAvailable SinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FRB-GFP11
Plasmid#181863PurposePlasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.DepositorInsertLyn-FRB-GFP11
UseTagsGFP11 and N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF NS34a-msGFP-'Q-mito
Plasmid#158764PurposeExpression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.DepositorInsertNS3/4a protease and msGFP
UseTagsExpressionMammalianMutationFlexible linkers between the protease and msGFP a…PromoterAvailable SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS1-mEGFP-NLS
Plasmid#205013PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-mCherry-NLS
Plasmid#205009PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49226PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/CGA-mEGFP-IRES-mCherry
Plasmid#49233PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDH_ss-SBP-EGFP
Plasmid#172358PurposeReporter to monitoring a streptavidin-binding peptide traffickingDepositorInsertER importing signal sequence
UseLentiviralTagsSBP-EGFPExpressionMammalianMutationPromoterCMVAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
AID-BFP-loxP_myo2_neoR
Plasmid#194054PurposeDual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genomeDepositorAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPP1_native+
Plasmid#67865Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form)DepositorInsertMRPP1 (TRMT10C Human)
UseTagsExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag2T-gRNA2
Plasmid#196253PurposePlasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sox17(2A-phiC31o)
Plasmid#140762PurposeTargeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.DepositorInsertphiC31o
UseMouse TargetingTagsExpressionMutationPromoterEndogenous Sox17Available SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/CGA-mEGFP-IRES-mCherry
Plasmid#49224PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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D44_Ld_180090
Plasmid#135858PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertalpha glucosidase II subunit, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLI_BFP_C-tag TNF
Plasmid#171187Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailable SinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sox1(2A-Cre)
Plasmid#140760PurposeTargeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.DepositorInsertCre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous Sox1Available SinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN D92A
Plasmid#50521PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
COG4 shRNA
Plasmid#163861PurposeshRNA targeting the coding sequences of COG4DepositorInsertcomponent of oligomeric golgi complex 4 (COG4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK206
Plasmid#110588PurposeBTK Type 3 coding sequence for golden gate assemblyDepositorInsertNanoluc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shCUX1-5328
Plasmid#100815PurposeLentiviral vector expressing a doxycycline-inducible short hairpin RNA against CUX1 sequence starting at 5328 of M74099DepositorInsertTGCTGTTGACAGTGAGCGTCTTCTCGTTTGAAACTTTGAATAGTGAAGCCA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-P(11)4
Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
UseTagsExpressionBacterialMutationPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIhh-5A9-LUC
Plasmid#46318DepositorInsert5 copies of A9 sequence from Ihh promoter
UseTagsluciferaseExpressionMammalianMutationPromotermouse osteocalcin gene 2 promoterAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-/UAA-mEGFP-IRES-mCherry
Plasmid#49225PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
RAMA-bio-His
Plasmid#50737PurposeExpresses enzymatically monobiotinylated full length RAMA ectodomainDepositorInsertRAMA
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_003
Plasmid#123817PurposeEncodes Nuclear import sequence as a Type 4a part to be used in the MTK systemDepositorInsertNLS
UseTagsExpressionMammalianMutationPromoterAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
UseTagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable SinceSept. 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits