We narrowed to 11,278 results for: VARS

1. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

   Plasmid

   #153540

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-F3UXG6

   Plasmid

   #103108

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-C7TEQ6

   Plasmid

   #103092

   Purpose

   Cas9 coding gene template with optimized sequence for human codon usage

   Depositor

   Duhee Bang

   Article

   Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

   Insert

   C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
   

   Use

   CRISPR; Cloning vector

   Mutation

   human codon-optimized

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Thy-Kv2.1-HA

   Plasmid

   #111541

   Purpose

   For expression of human KCNB1 in mouse brain

   Depositor

   Federico Sesti

   Article

   Yu et al J Neurosci. 2016 Oct 26;36(43):11084-110

   Insert

   thy1.2-Kv2.1-HA (KCNB1 Human)
   

   Use

   Mouse Targeting

   Tags

   HA

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_CD2AP-2/3

   Plasmid

   #91391

   Purpose

   Protein expression and purification of human SH3 domain construct CD2AP-2/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   CD2AP-2/3 (CD2AP Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. B-SpCas9

   Plasmid

   #126760

   Purpose

   Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   amino acids 1005-1013 replaced with two glycine

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVLAD6-mmPtch1-B-NNQ-TEV-SBP

   Plasmid

   #120887

   Purpose

   BacMam vector for Ptch1-B NNQ variant expression

   Depositor

   Philip Beachy

   Article

   Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

   Insert

   Ptch1 (Ptch1 Mouse)
   

   Tags

   SBP

   Expression

   Mammalian

   Mutation

   deleted amino acids 620-643, truncated at 1291, N…

   Promoter

   CMV

   Available Since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2058

   Plasmid

   #49136

   Purpose

   AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-CAGGS-emGFP-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mTFP1-Gamma-Tubulin-17

   Plasmid

   #55485

   Purpose

   Localization: Centrosomes, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid

    #114133

    Purpose

    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC809 - pAAV-EF1a-CRTsigpep-GCaMP3-KDEL

    Plasmid

    #63884

    Purpose

    An AAV packaging vector that expresses ER-retained GCaMP3 under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized GCaMP3(wt)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHH0103_CD2AP-1/3

    Plasmid

    #91398

    Purpose

    Protein expression and purification of human SH3 domain construct CD2AP-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    CD2AP-1/3 (CD2AP Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHH0103_EPS8-1/1

    Plasmid

    #91254

    Purpose

    Protein expression and purification of human SH3 domain construct EPS8-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    EPS8-1/1 (EPS8 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1958

    Plasmid

    #82569

    Purpose

    High copy number plasmid containing PR2/3:Con MiniPromoter insert.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    PRS2/3:Con
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Available Since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pHH0103_BAIAP2L2-1/1

    Plasmid

    #91393

    Purpose

    Protein expression and purification of human SH3 domain construct BAIAP2L2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BAIAP2L2-1/1 (BAIAP2L2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-D4J3S7

    Plasmid

    #103096

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS1978

    Plasmid

    #49110

    Purpose

    AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple251-icre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    C8ORF46

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pUC19-E3HCA8

    Plasmid

    #103100

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-A8REH9

    Plasmid

    #103084

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    A8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-J6LE60

    Plasmid

    #103117

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    J6LE60(Cas9 coding gene from Rhodovulum sp. PH10)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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