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hSyn1-AsCpf1(RR)

Plasmid

#109325

Purpose

AAV vector expressing AsCpf1 (RR variant)

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

hAsCpf1(RR)

Use

AAV

Tags

HA and NLS

Mutation

S542R/K607R

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del5

Plasmid

#32063

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-S752, insert V)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pEMS1943

Plasmid

#82565

Purpose

High copy number plasmid containing Ple266 (S100B MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple266

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1P POLD1_2

Plasmid

#160792

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-hIP-dCas9-BSD

Plasmid

#183231

Purpose

Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

Human insulin promoter

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPKm-105

Plasmid

#104853

Purpose

pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PhyB NT GBD

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR insertionH

Plasmid

#32067

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Insert H at H773-V774

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-shKcnk13

Plasmid

#120721

Purpose

Expresses GFP and an shRNA targeting Kcnk13 (in pLL3.7)

Depositor

Amy Lasek

Article

You et al Neuropharmacology. 2018 Oct 14;144:29-36

Insert

Kcnk13 (Kcnk13 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Promoter

mouse U6

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Promoter

Ple341

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_2

Plasmid

#160764

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_DRD1-NTEV-TCS-GV-2xHA

Plasmid

#194358

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD1-NTEV-TCS-GV-2xHA (DRD1 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS1977

Plasmid

#49109

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pBabe EGFR Del2

Plasmid

#32065

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete S752-I759

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pUC19-E6MBW6

Plasmid

#103102

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1P1084A-VSV

Plasmid

#139358

Purpose

Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Tags

VSV

Expression

Mammalian

Mutation

P1084A

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJJGL004

Plasmid

#180608

Purpose

Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX+Region1 insertion

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Insert

PlmCasX with DpbCasX R1 loop

Use

CRISPR

Tags

10xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

Plasmid

#184334

Purpose

Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_CRK-1/2

Plasmid

#91367

Purpose

Protein expression and purification of human SH3 domain construct CRK-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-1/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSRW1JZ

Plasmid

#129528

Purpose

C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

Depositor

Noelle L'Etoile

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Expression

Mammalian

Promoter

UGT8

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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