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pRibo-Sec-APEX2

Plasmid

#111699

Purpose

Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc R171H

Plasmid

#84671

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pMV306-ribo-APEX2

Plasmid

#111700

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (integrating)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCZ45ai

Plasmid

#16469

Depositor

Bert Vogelstein

Article

Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

Insert

p53 binding site (TP53 Human)

Expression

Yeast

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pGL3_ctrl_CP-candidate_luc+

Plasmid

#86392

Purpose

Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Luciferase

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRS303:VC

Plasmid

#37556

Depositor

Wei-Lih Lee

Article

Markus et al Cytoskeleton (Hoboken). 2011 Mar;68(3):1

Insert

Venus C-term (aa 155-238)

Expression

Bacterial

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX Rad

Plasmid

#11227

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1995 Mar 3. 270(9):4805-12.

Insert

Rad (RRAD Human)

Tags

GST

Expression

Bacterial

Mutation

N39: deletion of amino acids 1-39

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
His-TEV-U_(+36GFP)-Cre

Plasmid

#71750

Purpose

His-TEV-U+36GFP-Cre for E.Coli expression

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)-Cre

Tags

6X His and TEV protease

Expression

Bacterial

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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LexAop2->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87640

Purpose

For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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XE75 Beta-catenin Delta N-9 GFP

Plasmid

#16831

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin Delta N - 9 GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtAwoH

Plasmid

#65017

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

tetracycline promoter/operator

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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pES001 (pQacA-deGFP)

Plasmid

#69030

Purpose

Reporter Plasmid - deGFP driven by a qacR responsive promoter

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Insert

deGFP

Expression

Bacterial

Promoter

qacA

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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PAS529 (TJF072)

Plasmid

#65603

Purpose

pETDuet-1 Containing FadD Q338R-His

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD

Tags

6x His

Expression

Bacterial

Mutation

Q338R

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
PAS504 (TJF067)

Plasmid

#65571

Purpose

pETDuet-1 Containing FadD F447S

Depositor

Pamela Silver

Article

Ford et al PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717

Insert

FadD with F447S mutation

Expression

Bacterial

Mutation

F447S

Promoter

T7 lacO

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only
pMiR-HPV16-E1(partial)

Plasmid

#53699

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E1

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E1(partial)

Use

Luciferase

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only
pDR-meptrac-H194E

Plasmid

#47764

Purpose

expresses MepTrac-H194E in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

MepTrac-H194E

Tags

mcpGFP

Expression

Yeast

Mutation

H194E

Promoter

PMA

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only

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