We narrowed to 8,323 results for: gnal

1. pMG058 MBP-GS 634-666-His

   Plasmid

   #154077

   Purpose

   Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GYS1 (GYS1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNL-CEF-IgM

   Plasmid

   #187013

   Purpose

   Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6-N-3XFLAG-Fzd6

   Plasmid

   #123588

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   frizzled class receptor 6 (Fzd6 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5 Flag Grb10 9A long

   Plasmid

   #37485

   Depositor

   David Sabatini

   Article

   Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

   Insert

   Grb10 9A (GRB10 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   isoform A, 594aa, S104A S150A T155A S158A S426A S…

   Promoter

   Available Since

   Aug. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pRevTRE MKL1-N100 S449A/T450A/S454A

   Plasmid

   #19849

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1-N100 S449A/T450A/S454A (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mutation

   deleted amino acids 1-100 and changed Serine 449,…

   Promoter

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

   Plasmid

   #51257

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 216,220,224,230,248,789,805,817,848,9…

   Promoter

   EF1a

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

   Plasmid

   #123339

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCWXPGR-pTF-DVL2deltaDIX

    Plasmid

    #114276

    Purpose

    TET-inducible expression of DVL2 protein with a deletion of its DIX-domain

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    DVL2 (Dvl2 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    deletion of DIX domain

    Promoter

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid

    #51256

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 216,220,224,230,248 mutated to Glu

    Promoter

    EF1a

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCMV4Neo-murine IL-17RASEFIR-HA

    Plasmid

    #46863

    Purpose

    expresses mouse IL-17RA with SEFIR deletion

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RA-delta-SEFIR (Il17ra Mouse)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Deletion of SEFIR domain (AA 394-485)

    Promoter

    CMV

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

13. HMGN1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176943

    Purpose

    Mammalian expression vector encoding HMGN1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    HMGN1 (HMGN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPGK-T7/2-CD44v9 (-cyt)

    Plasmid

    #137819

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v9 (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCin4-3xFLAG-MKL1 S449A/T450A/S454A

    Plasmid

    #19845

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 S449A/T450A/S454A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449, Threonine 450 and Serine 454 …

    Promoter

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid

    #79886

    Purpose

    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123337

    Purpose

    Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Lyn-Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase, Ve…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-FLCN (C terminus)

    Plasmid

    #72297

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FLCN C-terminus (FLCN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains only C terminus of FLCN as defined in PM…

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid

    #123343

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5 beta4 1355T

    Plasmid

    #16038

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only