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pOBCol2.3-GFPemd

Plasmid

#110210

Purpose

Labels cells in the late osteoblast lineage.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPemerald

Tags

None

Expression

Mammalian

Promoter

2.3 kb rat type 1 collagen + first intron

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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inert_EJC

Plasmid

#216224

Purpose

GFP P2A RFP reporter with inert EJC immediately following stop codon

Depositor

Rebecca Voorhees

Article

Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

Insert

GFP P2A RFP_inert_EJC

Expression

Mammalian

Available Since

April 15, 2024

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

Plasmid

#71805

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneoRL-BCL2 3'UTR

Plasmid

#84597

Purpose

Renilla luciferase reporter containing BCL2 3' UTR

Depositor

Yanan Yang

Article

Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

Insert

BCL2 3'UTR (UTS2R Human)

Use

Luciferase

Tags

Renilla Luciferase

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only
pCyterm-mScarlet-I3_N1

Plasmid

#189777

Purpose

Construct for testing oligimerization of mScarlet-I3 in mammalian cells by OSER assay

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

Cyterm-mScarlet-I3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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p3VEA3

Plasmid

#234796

Purpose

Mammalian expression of bovine arrestin-3

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3230

Plasmid

#234102

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

FuGFP

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

Plasmid

#112006

Purpose

For co-expression of GCaMP6s with mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mKate2

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ210

Plasmid

#109329

Purpose

Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and bar gene for plant selection

Depositor

Yiping Qi

Article

Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

Type

Empty backbone

Use

Gateway compatible attr1-attr2 destination vector

Expression

Plant

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag

Plasmid

#231601

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under CMV promoter

Depositor

Marvin Tanenbaum

Article

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pABE-dGFP

Plasmid

#226863

Purpose

Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

EGFP

Expression

Mammalian

Mutation

A111V

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pABE-dGFP-gRNA

Plasmid

#226864

Purpose

Human gRNA expression vector targeting the A111V mutation of non-fluorescent EGFP in plasmid pABE-dGFP; for use with ABEs

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 Flag-NFRKB

Plasmid

#19789

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

V1200A

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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CFP_Rad_pcDNA4_HisMaxC

Plasmid

#41654

Depositor

Henry Colecraft

Article

Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 Ma

Insert

Rad, GTP binding proteins (RGK) (Rrad Mouse)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pfli1

Plasmid

#22413

Depositor

Nathan Lawson

Article

Lawson et al Development. 2001 Oct . 128(19):3675-83.

Insert

fli1 (fli1 Zebrafish)

Use

Riboprobe

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only
E47 2/5-pCS2

Plasmid

#20914

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E47 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
Flag-FOXO6

Plasmid

#153144

Purpose

Expression of Flag tagged human FOXO6 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXO6 (FOXO6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CAG-BGHpA

Plasmid

#92161

Purpose

For lncRNA overexpression (in trans)

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt4-V5

Plasmid

#35929

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation C (492) --> T

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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