165,528 results

1. pCW57.1 CMYC

   Plasmid

   #164145

   Purpose

   Doxycycline inducible expression of c-MYC

   Depositor

   Richard Possemato

   Insert

   MYC (MYC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Doxycycline inducible promoter

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHiUGE smFP-V5 donor (all-in-one) ORF2

   Plasmid

   #200388

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-mEGFP-CREB (S133A)

   Plasmid

   #137002

   Purpose

   mEGFP-CREB donor for G-CREB with S133A mutation

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

   Insert

   CREB1 (Creb1 Rat)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Changes Serine 133 into Alanine

   Promoter

   CMV

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDae079 (eMutaT7[transition])

   Plasmid

   #187622

   Purpose

   Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

   Depositor

   Seokhee Kim

   Article

   Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

   Inserts

   TadA-8e-T7RNAP
   PmCDA1-T7RNAP
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

   Plasmid

   #221550

   Purpose

   Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   gRNA targeting human TBK1 (TBK1 Human)
   

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

   Plasmid

   #83842

   Purpose

   Fluorescent probe for S/G2 cell cycle transition and G2/M transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCL151

   Plasmid

   #134455

   Purpose

   TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   linker-mScarlet-I for C-terminal fusion
   

   Expression

   Bacterial

   Promoter

   plac

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TetO-FUW-NICD

   Plasmid

   #61540

   Purpose

   Expresses NOTCH-ICD in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   NOTCH-ICD (NOTCH1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   NOTCH intracellular domain (aa1761-2555); A1943S…

   Available Since

   Jan. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0591

    Plasmid

    #142654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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