We narrowed to 38,497 results for: LAT

1. pSilencer2.1-U6-4JC1RR

   Plasmid

   #190847

   Purpose

   For mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSilencer2.1-U6-NL1F50

   Plasmid

   #190831

   Purpose

   For mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   NL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Flag-MCPIP1(1-555)

   Plasmid

   #90105

   Purpose

   Mammalian expression of Flag-MCPIP1(1-555)

   Depositor

   Mingui Fu

   Article

   Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

   Insert

   MCPIP1 (ZC3H12A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCHECK2-miR-34 MT

   Plasmid

   #78259

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CCGT to GG…

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET14b PHAS-I

   Plasmid

   #15679

   Depositor

   John Lawrence

   Article

   Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

   Insert

   PHAS-I (Eif4ebp1 Rat)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

   Plasmid

   #191627

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJB1-RPS22B-Cl

   Plasmid

   #160431

   Purpose

   Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

   Depositor

   Gary Stormo

   Article

   Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

   Insert

   RPS22B (RPS22B Budding Yeast)
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Removed introns, shuffled codons to change nucleo…

   Promoter

   GAL1 and S. cerevisiae GAL1

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TNF-SBP-mCherry_puromycin

   Plasmid

   #65285

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Use

   Tags

   GST and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Lentiviral CaMKKbeta

    Plasmid

    #33322

    Depositor

    Anthony Means

    Article

    Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

    Insert

    Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    human PGK

    Available since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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