Addgene

Showing: 5141 - 5160 of 157662 results

Results per page:

Sort results by:

pDD425

Plasmid

#202078

Purpose

Frame +0 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +0 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1 alpha and TRE

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RAR-TRE-mStrawberry reporter

Plasmid

#158683

Purpose

This plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET3a-hUBA1

Plasmid

#63571

Purpose

Expression of human UBA1 tagged with His

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBA1 (UBA1 Human)

Use

Tags

Expression

Bacterial

Mutation

Isoform 2 (1018 amino acids)

Promoter

T7

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Use

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-Neo-PIP-FUCCI

Plasmid

#118616

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
IST1-GFP

Plasmid

#186298

Purpose

Expresses human IST1 C-terminally fused to GFP

Depositor

Geert van den Bogaart

Article

Stempels et al J Cell Biol. 2023 Jul 3;222(7):e20220513

Insert

IST1 homolog (IST1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

GFP

Promoter

CMV

Available since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCBL101-RUBY

Plasmid

#199723

Purpose

Expresses betalain biosynthesis genes under CaMV 35S promoter

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

35S-RUBY

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-GFP-Sox11

Plasmid

#120387

Purpose

over expression of sox11 using infection

Depositor

Igor Ulitsky

Article

Perry et al Mol Cell. 2018 Nov 1;72(3):553-567.e5. d

Insert

sox11 (Sox11 Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-15F11-HA-mCh

Plasmid

#129591

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-inducible mCherry reporter

Plasmid

#64128

Purpose

Photoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetOx13-CMV

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV2_CAG_oROS-G

Plasmid

#216115

Purpose

Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.01.31.578117

Insert

oROS-G

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX-MCS-ASC-GFP

Plasmid

#73957

Purpose

Express human ASC with a C-terminal EGFP tag in mammalian cells

Depositor

Christian Stehlik

Article

de Almeida et al Immunity. 2015 Aug 18;43(2):264-76. doi:

Insert

PYD and CARD domain containing, PYCARD (PYCARD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CaV2.1 (pcDNA6)

Plasmid

#26578

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Use

Tags

His6 and V5

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TGFBR2

Plasmid

#116800

Purpose

Lentiviral expression of TGFBR2

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-fDIO-mCherry-WPREpA

Plasmid

#177328

Purpose

Control vector for Flp-dependent DREADD constructs

Depositor

Ulrik Gether

Insert

mCherry

Use

AAV; Flp-frt

Tags

Expression

Mammalian

Mutation

Promoter

Human Synapsin-1

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge