159,352 results

1. MAC_N_KLF6

   Plasmid

   #167772

   Purpose

   Expresses KLF6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF6 (KLF6 Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCF806-reci

   Plasmid

   #186711

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SNM-Luc

   Plasmid

   #35156

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 502 bp of the myc promoter

   Promoter

   c-myc

   Available since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pTF-ahpC-rfp

   Plasmid

   #153035

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAX APP 695

   Plasmid

   #30137

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type splice variant 695

   Promoter

   Available since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. dimericTomato-1xwGBD

   Plasmid

   #176099

   Purpose

   Visualization of active Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

   Insert

   WAS (WAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMVdel

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0058

   Plasmid

   #143007

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MAF (MAF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PASTE payload acceptor with attP site pDY2087

   Plasmid

   #219859

   Purpose

   Bxb1 payload vector with 5' attP

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKC96 pBABE H2B-mCherry

   Plasmid

   #192695

   Purpose

   Retroviral transgene expression of human H2B

   Depositor

   Iain Cheeseman

   Article

   Funk et al Cell. 2022 Nov 23;185(24):4634-4653.e22.

   Insert

   H2B
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2-hBIN1-V5

    Plasmid

    #27303

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pTSAR3.5t

    Plasmid

    #210257

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    eBFP2
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTSAR3.4t

    Plasmid

    #210256

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCherry
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRBN_ΔHBD

    Plasmid

    #232779

    Purpose

    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain

    Depositor

    Ivan Dikic

    Article

    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2

    Insert

    Cereblon (CRBN Human)
    

    Use

    Tags

    MBP-His6-TEV

    Expression

    Bacterial

    Mutation

    CRBN (residues 47 to 193 and 249 to 436) with GNG…

    Promoter

    T7

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nb-gp41-GFP (MoonTag-Nb-GFP)

    Plasmid

    #128602

    Purpose

    Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and sfGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_iETV2_P2A_GFP_Puro

    Plasmid

    #168805

    Purpose

    PiggyBac transposon construct with doxycycline inducible ETV2 + GFP expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ETV2 (ETV2 Synthetic, Human)
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNF02-mChartreuse

    Plasmid

    #219397

    Purpose

    Constitutive expression of mChartreuse in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mChartreuse
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCB1532H-RFP

    Plasmid

    #101855

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance with RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Article

    Pennington et al Figshare (2017)

    Type

    Empty backbone

    Use

    Fungal expression plasmid, tested in magnaporthe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFRT-DHX36iso1

    Plasmid

    #159585

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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