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We narrowed to 44,728 results for: cha;

Showing: 10281 - 10320 of 44728 results
  1. PtetA-PBAD-PLacO3O1 200bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid
    #225649
    Purpose
    tetA, araBAD and LacO3O1 promoters in tandem conformation with 200bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance
    Insert
    tetR/tetA, araBAD and LacO3O1 in tandem conformation
    Tags
    mCherry
    Expression
    Bacterial
    Promoter
    tetA, araBAD and LacO3O1 promoters
    Available Since
    Oct. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pEndo-I-OnuI-TSM

    Plasmid
    #207954
    Purpose
    Expresses a fusion of the homing endonuclease I-OnuI with the thermosensitive L212P VMA1 intein. Endonuclease activity is restricted to lower temperatures.
    Depositor
    Insert
    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Leucine 212 changed to proline, leucine 434 in fu…
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. PCUP1-GFP-Pho8-406

    Plasmid
    #207006
    Purpose
    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.
    Depositor
    Insert
    PHO8
    Tags
    GFP
    Expression
    Yeast
    Available Since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. PCUP1-GFP-Osh1-406

    Plasmid
    #207007
    Purpose
    Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.
    Depositor
    Insert
    OSH1
    Tags
    GFP
    Expression
    Yeast
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pASPIre4

    Plasmid
    #196656
    Purpose
    Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.
    Depositor
    Insert
    Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
    Expression
    Bacterial
    Mutation
    SpeI site in Bxb1 CDS
    Promoter
    rhamnose-inducible promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available Since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 10281 - 10320 of 44728 results