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pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Expression

Mammalian

Promoter

EF‐1α

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pRRL MND GFP

Plasmid

#36247

Depositor

David Rawlings

Article

Astrakhan et al Blood. 2012 Mar 19.

Insert

GFP (eGFP )

Use

Lentiviral

Promoter

MND

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Article

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

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CpxR-GFP

Plasmid

#220150

Purpose

To track the natural CpxR promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

CpxR promoter only

Expression

Bacterial

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJR152

Plasmid

#196280

Purpose

Cassette used in dual sgRNA library generation

Depositor

Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Insert

hU6-sgRNA-CR3

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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PNCA-GFP-dYY1

Plasmid

#67791

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

Insert

MMLV-LTR-GFP

Mutation

Deletion of LTR U3 YY1 binding domain

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pRG646_lexO-HO_LexA-TF_LEU2MX

Plasmid

#154815

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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PKNOX1_pCSdest

Plasmid

#53899

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PKNOX1 (PKNOX1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TREER-Actin

Plasmid

#208330

Purpose

TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TREER-Actin

Tags

TREER

Expression

Mammalian

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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