We narrowed to 40,651 results for: tro

1. pCS6-SMASh-YFP

   Plasmid

   #68852

   Purpose

   Expresses SMASh tag fused to YFP at N terminus in mammalian cells

   Depositor

   Michael Lin

   Article

   Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

   Insert

   SMASh-YFP
   

   Use

   Tags

   SMASh tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMX229

   Plasmid

   #23002

   Depositor

   Linda Wordeman

   Article

   Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

   Insert

   Kif18A (KIF18A Human)
   

   Use

   Tags

   6His and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFlpStop-attB-UAS-2.1-tdTom

   Plasmid

   #88910

   Purpose

   attB FlpStop plasmid, for insertion into MiMIC site

   Depositor

   Tom Clandinin

   Article

   Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

   Insert

   FlpStop
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   5XUAS

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-anti-mCherry(LaM6) PAGER(FL)

   Plasmid

   #230023

   Purpose

   Expresses anti-mCherry PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-LaM6-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. human MMP-2 promoter

   Plasmid

   #53432

   Purpose

   promoter activity study

   Depositor

   Etty Benveniste

   Article

   Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

   Insert

   MMP2 promoter (MMP2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   MMP2

   Available since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFAP-Arclight

   Plasmid

   #187978

   Purpose

   Expresses the fluorescent voltage indicator Arclight under the GFAP promoter

   Depositor

   Chris Dulla

   Article

   Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

   Insert

   Arclight A242
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRN3P_membrane_EGFP

   Plasmid

   #139402

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   membrane-EGFP
   

   Use

   In vitro transcription

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEM705 Gsi (Ser)

   Plasmid

   #109352

   Purpose

   GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gsi chimera
   

   Use

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cys at position 391 to Serine (Pertussis …

   Promoter

   CAG

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNMT

   Plasmid

   #42578

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   N-myristoyl tranferase (NMT1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two silent mutations T690C and T942C

   Promoter

   T7

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-N-I-N-CAAX

    Plasmid

    #41686

    Purpose

    Expresses caged Cdc42 GEF intersectin: Intersectin Dbl homology (DH) domain fused to Dronpa145N at C terminus and at N terminus, for optogenetic activation

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145N-IntersectinDH-Dronpa145N-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    K145N on Dronpa

    Promoter

    CMV

    Available since

    Dec. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBABE-Stat5a1*6

    Plasmid

    #130668

    Purpose

    Retroviral vector for the expression of constitutive Stat5a (puromycin resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

    Insert

    Stat5a (Stat5a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Histidine 298 to Arginine and Serine 710 …

    Promoter

    LTR

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CRY2low-tdTomato

    Plasmid

    #104067

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 gfaABC1D-cyto-GCaMP6f

    Plasmid

    #52925

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Baljit Khakh

    Article

    Haustein et al Neuron. 2014 Apr 16;82(2):413-29. doi: 1

    Has Service

    AAV5

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-6xHis-(-30)GFP

    Plasmid

    #62936

    Purpose

    Expression of 6xHis-(-30)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (-30)GFP
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDY1243-Fluorescent RADARS

    Plasmid

    #182540

    Purpose

    Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    mNeon
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-hisMBP-AsCpf1-EC

    Plasmid

    #79007

    Purpose

    Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    AsCpf1
    

    Use

    Tags

    6xHis-MBP-NLS and NLS-HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psiCHECK-2-Smad2-3'UTR

    Plasmid

    #61775

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. psiCHECK-2-Smad5-3'UTR

    Plasmid

    #61777

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGEMHE-H2B-mEGFP

    Plasmid

    #105528

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    H2B (H2BC11 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPEY-GAL-Kan

    Plasmid

    #232099

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRISPEY-GAL-Hyg

    Plasmid

    #232098

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21d-myc-BirA

    Plasmid

    #109424

    Purpose

    Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    BirA (birA )
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTalpha1-iCre

    Plasmid

    #133924

    Purpose

    Neuron-specific expression of iCre

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    iCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    neuron-specific tubulin-alpha-1 promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PGAL

    Plasmid

    #60337

    Purpose

    Contains full length native galactose promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Inserts

    promoter
    yECitrine
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CAG-Blimp1

    Plasmid

    #73995

    Purpose

    Plasmid expressing mouse Blimp1

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    Blimp1 gene (Prdm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZR060_CROPseq-sgCtrl-CRISPRi

    Plasmid

    #180269

    Purpose

    Lentiviral CROPseq vector expressing non-targeting control sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

    Plasmid

    #203770

    Purpose

    For production of template RNA for use in PRINT, mCherry under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for mCherry
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMVV108

    Plasmid

    #215338

    Purpose

    Expresses sfGFP under induction with the Salicylic Acid Marionette Cassette (NahRAM + PSalTTC-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PSalTTC

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMN-333

    Plasmid

    #195467

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF3 BS - ybTATA - mCherry] - pSFFV - ZF3 - p65 - ERT2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA-mCherry-4A/4B-CAAX

    Plasmid

    #87699

    Purpose

    Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    mCherry-4A/4B-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. fos-tTA

    Plasmid

    #34856

    Depositor

    Mark Mayford

    Article

    Reijmers et al Science. 2007 Aug 31;317(5842):1230-3.

    Insert

    Fos promoter/first intron -764/+918 (Fos Mouse)
    

    Use

    Mouse Targeting

    Tags

    Fos 3' UTR (~1500bp) and tTA (tetracycline-c…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDAS12395_PEAR-GFP_2.0-preedited

    Plasmid

    #177185

    Purpose

    PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBMN-HA-NAP1

    Plasmid

    #208868

    Purpose

    For mammalian expression of NAP1

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al bioRxiv September 25, 202

    Insert

    NAP1 (AZI2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLV 4xSTAR-sTomato-NLS-blast

    Plasmid

    #136259

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

    Insert

    stem cell Ascl2 reporter, 4 repeats
    

    Use

    Lentiviral

    Tags

    sTomato-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJMP8823

    Plasmid

    #220901

    Purpose

    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PC-dCas9-novo

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    dCas9-novo
    

    Use

    CRISPR

    Tags

    3xMyc

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBDC

    Plasmid

    #135188

    Purpose

    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    CmR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge