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We narrowed to 13,837 results for: Cre

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Showing: 5161 - 5180 of 13837 results

  1. pLentiRNACRISPR_001 - hU6-DR_BsmBI-EFS-PguCas13b-NLS-2A-Puro-WPRE

    Plasmid
    #138143
    Purpose
    Expresses PguCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac
    Depositor
    Neville Sanjana
    Publication
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    PguCas13b
    Use
    Lentiviral
    Tags
    NLS
    Available since
    March 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138143-plasmid-map-sequence-id-275400

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  3. pDualIN-SMN2

    Plasmid
    #214430
    Purpose
    Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7
    Depositor
    Chaolin Zhang
    Publication
    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMN2 EXON 7 (SMN2 Human)
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    214430-plasmid-map-sequence-id-424358

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  8. TFORF2207

    Plasmid
    #143354
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB3L2 (CREB3L2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143354-plasmid-map-sequence-id-400312

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  9. PX458_ATF4_1

    Plasmid
    #72351
    Purpose
    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATF4 (ATF4 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    72351-plasmid-map-sequence-id-132101

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  12. pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE

    Plasmid
    #123362
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase.
    Depositor
    Sidi Chen
    Publication
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    Firefly luciferase
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123362-plasmid-map-sequence-id-240467

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  16. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Publication
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Available since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  20. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

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Showing: 5161 - 5180 of 13837 results