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pSB1C3_mTq2-Tat_YPet-PCP (cy-plasmid)

Plasmid

#127636

Purpose

Encodes two fluorecent proteins with RNA binding peptides: mTurquoise2-Tat and YPet-PCP. Expression with constitutive E. coli RNAP promoter (J23106), ribozymes RiboJ10 (for YPet) and PlmJ (for mTq2)

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

Insert

mTurquoiuse2-Tat, YPet-PCP

Use

Synthetic Biology

Tags

RNA binding peptide: PCP and RNA binding peptide:…

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2367

Plasmid

#225636

Purpose

High Copy number plasmid containing Ple384 (TH MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Galvan et al Sci Rep. 2026 Feb 16. doi: 10.1038/s4159

Insert

Ple384 (TH Human)

Use

Unspecified

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-CSPG5 12959 (VL + VH) synNotch

Plasmid

#247513

Purpose

Expresses a synNotch receptor binding to CSPG5

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to CSPG5

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PGK- CDH10#1-synNotch-Gal4VP64

Plasmid

#247503

Purpose

Expresses a synNotch binding to CDH10 (cadherin 10)

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

synNotch receptor using a scFvs binding to CDH10 (cadherin 10)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PGK- CDH10#3-synNotch-Gal4VP64

Plasmid

#247504

Purpose

Expresses a synNotch binding to CDH10 (cadherin 10)

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

synNotch receptor using a scFvs binding to CDH10 (cadherin 10)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-CSPG5 12959 (VH+VL) synNotch

Plasmid

#247514

Purpose

Expresses a synNotch receptor binding to CSPG5

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to CSPG5

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA domain 13058 (VH+VL) synNotch

Plasmid

#247518

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-CSPG5 12963 (VH+VL) synNotch

Plasmid

#247516

Purpose

Expresses a synNotch receptor binding to CSPG5

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to CSPG5

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-CSPG5 12963 (VL+VH) synNotch

Plasmid

#247515

Purpose

Expresses a synNotch receptor binding to CSPG5

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to CSPG5

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ anti-PTPRZ CA domain 13059 (VH+VL) synNotch

Plasmid

#247520

Purpose

Expresses a synNotch receptor binding to PTPRZ

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to PTPRZ

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ GRM3 13144 VLVH synNotch

Plasmid

#247571

Purpose

Expresses a synNotch receptor binding to GRM3

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to GRM3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_ gb128_ BCAN 13138 VLVH synNotch

Plasmid

#247573

Purpose

Expresses a synNotch receptor binding to BCAN

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

Expresses a synNotch receptor binding to BCAN

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTE3: pFuGW-SSX1p-GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS

Plasmid

#240456

Purpose

A synthetic transcription factor (synTF) that contains concensus 3CLpro cleavage site (CS)

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-1X3CLpro consensus CS-VP16AD-SV40NLS

Use

Lentiviral

Promoter

SSX1 and SSX1p

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSE163: pFuGW-SSX1p-GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

Plasmid

#240457

Purpose

A synthetic transcription factor (synTF) that contains a synthetic general 3CLpro cleavage site (CS)

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-1X3CLpro synthetic general CS -VP16AD-SV40NLS

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

Plasmid

#240460

Purpose

a synthetic transcription factor (synTF) that contains 4 tandem synthetic general 3CLpro cleavage site (CS).

Depositor

Lior Nissim

Article

Edri et al Cell Rep Methods. 2025 Aug 5:101139. doi

Inserts

SSX1p
GAL4BD-4X3CLpro synthetic general CS-VP16AD-SV40NLS

Use

Lentiviral

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 CDS A. thaliana MAMI

Plasmid

#209186

Purpose

pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT5G54110.1 (MAMI Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA2

Plasmid

#185007

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA2

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA1

Plasmid

#185002

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA1 (Akp3 Mouse)

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMERVK9C-del-gRNA1

Plasmid

#185004

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMERVK9C-del-gRNA1

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA1

Plasmid

#185006

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA1

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMERVK9C-del-gRNA2

Plasmid

#185005

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMERVK9C-del-gRNA2

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA2

Plasmid

#185003

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA2 (Akp3 Mouse)

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-U6-UL29-U3-UL8

Plasmid

#166685

Purpose

To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

Depositor

Yujia Cai

Article

Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

Insert

UL8 and UL29 gRNA (UL29, UL8 HSV-1)

Use

Lentiviral

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_2_dCas9_VP64_sgRNA

Plasmid

#163707

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
VP64+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2046

Plasmid

#79654

Purpose

ssAAV genome with Ple257 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple257-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1942

Plasmid

#105867

Purpose

High copy number plasmid containing Ple265 (PCP2 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

Insert

Ple265

Promoter

Ple265 (PCP2 MiniPromoter)

Available Since

Feb. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLNCX2-mCherry-CHMP2B-siRNAres

Plasmid

#115333

Purpose

expresses siRNA-resistant mCherry-CHMP2B in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2B (CHMP2B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS1930

Plasmid

#79779

Purpose

High copy number pUC vector containing Ple257 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple257

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple257 (PAX6 MiniPromoter)

Available Since

Nov. 15, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2047

Plasmid

#79655

Purpose

ssAAV genome with Ple258 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple258-EmGFP WPRE

Use

AAV

Promoter

PAX6 Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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