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pET28a-OuIAD(H514E)

Plasmid

#226972

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514E variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514E)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514E

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(H514A)

Plasmid

#226971

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) H514A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(H514A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

H514A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(C500S)

Plasmid

#226970

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) C500S variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(C500S)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

C500S

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD(G853A)

Plasmid

#226969

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) G853A variant in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD(G853A)

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

G853A

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 I88A wgMDH

Plasmid

#204249

Purpose

Bacterial expression of I88A wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

I88A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 R196A wgMDH

Plasmid

#204271

Purpose

Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R196A

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 R196E wgMDH

Plasmid

#204272

Purpose

Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R196E

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 R130Q wgMDH

Plasmid

#204268

Purpose

Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

R130Q

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQE60 M66L wgMDH

Plasmid

#204247

Purpose

Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

M66L

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 M91Q wgMDH

Plasmid

#204250

Purpose

Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

M91Q

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE60 D87L wgMDH

Plasmid

#204248

Purpose

Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Use

Tags

6X His

Expression

Bacterial

Mutation

D87L

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPop2a-4xmut-dsRNAres-V5His6_R

Plasmid

#147435

Purpose

Insect Expression of DmPop2a-4xmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmPop2a-4xmut-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPop2a-5xmut-dsRNAres-V5His6_R

Plasmid

#147436

Purpose

Insect Expression of DmPop2a-5xmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmPop2a-5xmut-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPop2a-C80EL84E-dsRNAres-V5His6_R

Plasmid

#147437

Purpose

Insect Expression of DmPop2a-C80EL84E-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmPop2a-C80EL84E-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPop2a-C80EL84EE151A-dsRNAres-V5His6_R

Plasmid

#147438

Purpose

Insect Expression of DmPop2a-C80EL84EE151A-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmPop2a-C80EL84EE151A-dsRNAres (Pop2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-DmCCR4_1-166_R

Plasmid

#147439

Purpose

Bacterial Expression of DmCCR4_1-166

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCCR4_1-166 (twin Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-DmNot1_1710-2505_R

Plasmid

#147440

Purpose

Bacterial Expression of DmNot1_1710-2505

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1710-2505 (Not1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_402-436_R

Plasmid

#147420

Purpose

Insect Expression of DmNot2_402-436

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_402-436 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_436-585_R

Plasmid

#147421

Purpose

Insect Expression of DmNot2_436-585

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_436-585 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_453-585_R

Plasmid

#147422

Purpose

Insect Expression of DmNot2_453-585

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_453-585 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_469-585_R

Plasmid

#147423

Purpose

Insect Expression of DmNot2_469-585

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_469-585 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_478-585_R

Plasmid

#147424

Purpose

Insect Expression of DmNot2_478-585

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_478-585 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot3_677-844_I

Plasmid

#146611

Purpose

Insect Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot3_677-844 (Not3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot3_677-844_I

Plasmid

#146620

Purpose

Insect Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot3_677-844 (Not3 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2_402-585_I

Plasmid

#146608

Purpose

Insect Expression of DmNot2_402-585

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot2_402-585 (Rga Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNHA-C1-HsDCP1b_H

Plasmid

#146505

Purpose

Mammalian Expression of HsDCP1b

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1b (DCP1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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