We narrowed to 11,977 results for: pcDNA

1. pCDNA5/FRT_CMV_NES-Kinprola_AMPK_T/A-mEGFP

   Plasmid

   #233362

   Purpose

   CMV driven expression of the inactive AMPK activity recorder negative control with T/A mutation Kinprola_AMPK_T/A in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

   Insert

   NES-Kinprola_AMPK_T/A-mEGFP
   

   Use

   Tags

   NES and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-PDGFRA-K627M-V5/HIS

   Plasmid

   #234755

   Purpose

   Expression of the K627M kinase defective mutant variant of human PDGFRA

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA-K627M receptor tyrosine kinase, full length (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   K627M substitution

   Promoter

   CMV

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3

   Plasmid

   #233062

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Alfa and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt

   Plasmid

   #233240

   Purpose

   Parallel coiled-coil with (GGS)3 linker to prone condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)3-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt

   Plasmid

   #233241

   Purpose

   Parallel coiled-coil with (GGS)12 linker to prevent condensation

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-clover-GCN4-(GGS)12-Vt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 FFSS-BACH1 C122E

   Plasmid

   #232274

   Purpose

   Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) with Cys122 to Glu point mutation in human cell lines

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   changed cysteine 122 to glutamic acid

   Promoter

   CMV

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4TO T21R-T2A-mCherry

   Plasmid

   #225585

   Purpose

   Mammalian expression vector for Doxycycline inducible expression of TRIM21 RING with a self-cleaving T2A-mCherry fluorescent expression reporter

   Depositor

   William McEwan

   Article

   Benn et al Science. 2024 Aug 30;385(6712):1009-1016

   Insert

   T21R-T2A-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3puro-mGFP-FXR1-N2 (1-399)

   Plasmid

   #225452

   Purpose

   Express mEGFP-fusion protein of FXR1 fragment (1-399)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N2 fragment (1-399)

   Promoter

   CMV

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3puro-mGFP-FXR1-N1 (1-379)

   Plasmid

   #225450

   Purpose

   Express mEGFP-fusion protein of FXR1 fragment (1-379)

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FXR1 (FXR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N1 fragment (1-379)

   Promoter

   CMV

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Flag-Jarid2-polyA

    Plasmid

    #223811

    Purpose

    IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

    Depositor

    Azusa Inoue

    Article

    Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

    Insert

    Jarid2 (Jarid2 Mouse)
    

    Use

    In vitro transcription

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

    Plasmid

    #225464

    Purpose

    Express mEGFP-fusion protein of FMR1 fragment (1-445)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N1 fragment (1-445)

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3puro-mGFP-FMR1iso1-N2(1-491)

    Plasmid

    #225465

    Purpose

    Express mEGFP-fusion protein of FMR1 fragment (1-491)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    N2 fragment (1-491)

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5 FRT TO GFP11-LRRK2

    Plasmid

    #231174

    Purpose

    Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

    Depositor

    Samara Reck-Peterson

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    GFP11-LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(-)-EGFP-AP4-cyto

    Plasmid

    #229694

    Purpose

    Transient expression of EGFP AP4-cyto in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (cytoplasm)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-)-EGFP-FP4-cyto

    Plasmid

    #229695

    Purpose

    Transient expression of FP4-cyto in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

    Plasmid

    #228533

    Purpose

    A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:FRB:gs10:Cul1(dC501) (CUL1 Human, Synthetic)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

    Plasmid

    #228534

    Purpose

    Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO_eGFP-pScaE

    Plasmid

    #228826

    Purpose

    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaE in HeLa Flp-In cells

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaE
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 49-494

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_eGFP-pScaD

    Plasmid

    #228825

    Purpose

    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaD in HeLa Flp-In cells

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaD
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 30-700

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO_eGFP-pScaC

    Plasmid

    #228824

    Purpose

    For the expression of the passenger domain of the Orientia tsutsugamushi protein ScaC in HeLa Flp-In cells

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    aa 33-223

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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