We narrowed to 162,717 results for: addgene

1. pCJ1023

   Plasmid

   #228763

   Purpose

   Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGK65

   Plasmid

   #228123

   Purpose

   Express node 1795 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1550, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGK59

   Plasmid

   #228119

   Purpose

   Express node 1429 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1429, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGK57

   Plasmid

   #228117

   Purpose

   Express node 482 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 482, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGK56

   Plasmid

   #228116

   Purpose

   Express node 412 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 412, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGK60

   Plasmid

   #228120

   Purpose

   Express node 1438 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1438, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGK55

   Plasmid

   #228114

   Purpose

   Express node 2 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 2, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PCNT

   Plasmid

   #227284

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of PCNT (PCNT Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX401 APE1 CI

   Plasmid

   #227191

   Purpose

   Inducible lentiviral expression of CI (Catalytically Inactive) APE1

   Depositor

   David Pellman

   Article

   Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

   Insert

   apurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   H309N

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. dCas9 fused to scFv (anti-spike)

    Plasmid

    #186421

    Purpose

    Expression of dCas9 with C-terminal nanobody fusion recognizing spike protein from SARS-CoV-2

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    dCas9-scFv fusion (anti-SARS-CoV-2 spike)
    

    Use

    CRISPR

    Tags

    6His

    Expression

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVUT-spC3GFP

    Plasmid

    #225487

    Purpose

    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Secretable and permeable exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251

    Promoter

    Human ubiquitin C

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3xMS2 TR knockin sgRNA 1

    Plasmid

    #207607

    Purpose

    sgRNA for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    cgactcgcccggcagcgcac
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYSD_lambda_mRFP

    Plasmid

    #217831

    Purpose

    Yeast surface display Fab vector with lambda light chain and mRFP

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYSD_kappa_mRFP

    Plasmid

    #217830

    Purpose

    Yeast surface display Fab vector with kappa light chain and mRFP

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMP_lambda

    Plasmid

    #217829

    Purpose

    Mini-mutagenesis plasmid for lambda light chain YSD Fabs

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMP_kappa

    Plasmid

    #217828

    Purpose

    Mini-mutagenesis plasmid for kappa light chain YSD Fabs

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3xMS2 TR knockin sgRNA 2

    Plasmid

    #207608

    Purpose

    sgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    acccccaaacctgactgact
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GAL4(1-147)

    Plasmid

    #195029

    Purpose

    The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

    Depositor

    Andrew Lassar, Cheng-Hai Zhang

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    GAL4 (1-147)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-VN155(I152L)-SRCR5

    Plasmid

    #207993

    Purpose

    BiFC assay

    Depositor

    Young Tang

    Article

    Huang et al Virol J. 2020 Jul 30;17(1):116. doi: 10.

    Insert

    SRCR5 (CD163 pig)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

    Plasmid

    #209016

    Purpose

    Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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