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pDONR223-MAPK8

Plasmid

#23420

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK8 (MAPK8 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMig (Martins Lab)

Plasmid

#14125

Depositor

L. Miguel Martins

Article

Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

Insert

IRES-EGFP

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

Cla I sites were silenced in the original pRetroS…

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pET26b-Granzyme A (S-A)

Plasmid

#8825

Depositor

Judy Lieberman

Article

Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

Insert

Human Granzyme A inactive (GZMA Human)

Tags

(His)6

Expression

Bacterial

Mutation

changed Serine 184 to Alanine

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only
pSMPUW_6.14_M4_Ef1a-HA-gateway-SV40-Zeo

Plasmid

#128450

Purpose

lentiviral destination vector with Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB116

Plasmid

#68570

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti_9xD3B_Cluc_Norm

Plasmid

#68414

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Lentiviral and Luciferase

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available Since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7g

Plasmid

#51381

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7g (MIRLET7G Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH BRCC36

Plasmid

#27496

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

BRCC36 (BRCC3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLH116

Plasmid

#22651

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1987 Mar . 84(

Insert

Proteolipid Protein (Plp1 Mouse)

Use

Cloning vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pDmelOR-mRho.V5.mER.hOr47a

Plasmid

#126478

Purpose

Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Inserts

Or47a (Or47a Fly)
Orco (Orco Fly)

Tags

H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

Expression

Mammalian

Mutation

codon optimization for H. sapiens and codon optim…

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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Lentiviral CaMKKbeta K193A

Plasmid

#33323

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Mutation

K194A

Promoter

human PGK

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pcmv3-IRG1-HA

Plasmid

#198181

Purpose

Mammalian expression of HA-tagged human ACOD1 (IRG1)

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

ACOD1 (ACOD1 Human)

Tags

HA

Expression

Mammalian

Mutation

HA tag is added to C-terminal of target protein

Promoter

CMV

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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p413TEF-SirT1

Plasmid

#51746

Purpose

overexpression of human SirT1 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Article

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

SirT1 (SIRT1 Human)

Expression

Yeast

Promoter

TEF1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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MYC-NKX3.1

Plasmid

#42895

Depositor

Edward Gelmann

Article

Markowski et al Cancer Res. 2008 Sep 1;68(17):6896-901.

Insert

NKX3.1 (NKX3-1 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
MMTV-GAL4-SV40

Plasmid

#19255

Depositor

Philip Leder

Article

Ornitz et al Proc Natl Acad Sci U S A 1991 Feb 1;88(3

Insert

GAL4

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCMV_NESPylRS(AF)_hU6tRNAPyl

Plasmid

#223508

Purpose

PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

Depositor

Edward Lemke

Article

Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

Inserts

NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
tRNA Pyl

Expression

Mammalian

Mutation

Y306A; Y384F

Promoter

CVM, SP6 and U6

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR4-V5/HIS

Plasmid

#201109

Purpose

expression of human FGFR4 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pES002 MBP-GSK3β_S9A-HA-His

Plasmid

#196184

Purpose

Expresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Insert

GSK3B (GSK3B Human)

Tags

HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A Δ450-572-pBabe-Puro

Plasmid

#25958

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-HA-Slc1a5 (mouse)

Plasmid

#71458

Purpose

inducible expression fo Slc1a5 in mammalian cells

Depositor

Shao-Cong Sun

Article

Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

Insert

Slc1a5 (Slc1a5 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

A85P, L125F and V384A mutations compared to GenBa…

Promoter

CMV

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only