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RCAS DNhRARalpha (CC#1865)

Plasmid

#15153

Depositor

Connie Cepko

Publication

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

RAR alpha (dom neg) (RARA Human)

Use

Retroviral; Avian expression

Mutation

Dominant negative. Truncated at aa403, so lacks t…

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pYPQ141C

Plasmid

#69292

Purpose

Gateway entry vector; single gRNA under OsU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pICE-EGFP-FLAG-PSMD2

Plasmid

#161917

Purpose

Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Publication

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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HA-AR-LBD

Plasmid

#171239

Purpose

Mammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)

Depositor

Steven Balk

Publication

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform5

Plasmid

#70177

Purpose

Mammalian expression of hsOPA1 isoform 5

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 5 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Rat Alpha2 delta-1 pMT2

Plasmid

#58726

Purpose

expression of rat alpha2 delta-1 in mammalian cells

Depositor

Annette Dolphin

Publication

Davies et al Proc Natl Acad Sci U S A. 2010 Jan 26;10

Insert

alpha2 delta-1 (Cacna2d1 Rat)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 23, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Publication

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLKO.1-puro U6 sgRNA Oct4A -158

Plasmid

#50922

Purpose

U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Publication

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Oct4A -158 sgRNA (POU5F1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Publication

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUB_smFLAG_ActB_MS2

Plasmid

#81083

Purpose

Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Publication

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_ActB_24xMS2

Expression

Mammalian

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pYPQ135A

Plasmid

#69277

Purpose

Golden Gate entry vector; 5th gRNA under AtU6 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryBCL11B-W

Plasmid

#208758

Purpose

Lentiviral vector expressing mCherry fused with BCL11B cDNA

Depositor

Kosuke Yusa

Publication

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B (BCL11B Human)

Use

Lentiviral

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP7 (catalytic domain)

Plasmid

#16360

Depositor

Guy Salvesen

Publication

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP7 catalytic domain (SENP7 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 640 to 984 catalytic domain of human S…

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pME-QF

Plasmid

#61375

Purpose

Middle Gateway Entry Vector Containing QF

Depositor

Marnie Halpern

Publication

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QF

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDSG310

Plasmid

#115611

Purpose

surface-displayed Ag2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CIB-VP64 (B1016)

Plasmid

#92037

Purpose

Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

Depositor

Chandra Tucker

Publication

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1-VP64 (CIB1 Mustard Weed)

Tags

Fusion of plant CIB1 with VP64 activation domain.…

Expression

Mammalian

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CRY-Gal∆DD (B1013)

Plasmid

#92035

Purpose

Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

Depositor

Chandra Tucker

Publication

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

Fusion of plant CRY2 to Gal4 binding domain (AA1-…

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

Plasmid

#87803

Purpose

Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Publication

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcsII-pb4-flag

Plasmid

#86768

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Publication

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 4 (PSMB4 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Promoter

EF-1a

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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