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We narrowed to 5,233 results for: puc

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Showing: 5221 - 5233 of 5233 results

  1. pZNF445-donor

    Plasmid
    #112332
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF445 with GFP
    Depositor
    Kevin White
    Insert
    ZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
    Use
    CRISPR
    Mutation
    rs78585428, hg38:3:44446218:C>T, hg38:3:444466…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112332-plasmid-map-sequence-id-228561

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  5. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid
    #141100
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    Use
    Donor template
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141100-plasmid-map-sequence-id-292288

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  8. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  9. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  10. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

    Plasmid
    #197599
    Purpose
    Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive
    Depositor
    Alexander Gottschalk
    Article
    Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:
    Insert
    Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)
    Expression
    Worm
    Mutation
    D387A, E490G
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    197599-plasmid-map-sequence-id-410713

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  11. pMamCol1

    Plasmid
    #215184
    Purpose
    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli
    Depositor
    Krishna Kishore Inampudi
    Insert
    tsPurple
    Use
    AAV, CRISPR, and Synthetic Biology ; Recombinant …
    Tags
    SNAC-StrepII tag
    Expression
    Bacterial and Mammalian
    Promoter
    TetON 3G Bidirectional for Mammalian and Trc with…
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    215184-plasmid-map-sequence-id-430375

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  12. pMT1219

    Plasmid
    #228301
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    Use
    Synthetic Biology
    Tags
    GGG linker followed by His6 and MF_ secretion sig…
    Expression
    Yeast
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228301-plasmid-map-sequence-id-457688

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Showing: 5221 - 5233 of 5233 results