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pTR-342 pUC19-tNGFR-P2A-CD28 N

Plasmid

#186056

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CD28 (CD28 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTR-449 tNGFR-P2A-CCR2

Plasmid

#186057

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

CCR2 (CCR2 Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 TAPBPR-TM-TN6

Plasmid

#178650

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

E205K, R207E, Q209S, Q272S, switched transmembran…

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-R/G-ICUE

Plasmid

#181849

Purpose

ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

R/G-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d200

Plasmid

#114047

Purpose

Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d200 (TTK Human)

Expression

Mammalian

Mutation

delta 200 (deletion of NTE and TPR domains)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-fbp_RiboJ_GFP+_MGApt_Bba_B0015

Plasmid

#107578

Purpose

B. megaterium DSM319 fbp promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)

Depositor

Paul Freemont

Article

Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p

Insert

GFP+

Use

Synthetic Biology

Tags

B. megaterium DSM319 xylA leader sequence (MTSSKI…

Expression

Bacterial

Mutation

F64L/ S65T/ Q80R/ F99S/ M153T/ V163A

Promoter

fbp promoter Bacillus megaterium DSM319

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSN29

Plasmid

#100114

Purpose

expresses a chimeric cMYC-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCerulean-P2A-cMYC fused to full length HP1 alpha (MYC, CBX5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSN17

Plasmid

#100104

Purpose

expresses a chimeric OCT4-repressor fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to full length HP1 alpha (POU5F1, CBX5 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KIR3DL2_p.R96Q

Plasmid

#81444

Purpose

Gateway Donor vector containing KIR3DL2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KIR3DL2 (KIR3DL2 Human)

Use

Gateway entry vector

Mutation

R96Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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ADPGK gRNA (BRDN0001487160)

Plasmid

#77997

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADPGK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADPGK (ADPGK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADPGK gRNA (BRDN0001145310)

Plasmid

#77998

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADPGK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADPGK (ADPGK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADPGK gRNA (BRDN0001147802)

Plasmid

#77999

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADPGK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADPGK (ADPGK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADPGK gRNA (BRDN0001147330)

Plasmid

#78000

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADPGK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADPGK (ADPGK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GLYCTK gRNA (BRDN0001487112)

Plasmid

#77905

Purpose

3rd generation lentiviral gRNA plasmid targeting human GLYCTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GLYCTK (GLYCTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GLYCTK gRNA (BRDN0001147448)

Plasmid

#77906

Purpose

3rd generation lentiviral gRNA plasmid targeting human GLYCTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GLYCTK (GLYCTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GLYCTK gRNA (BRDN0001146438)

Plasmid

#77907

Purpose

3rd generation lentiviral gRNA plasmid targeting human GLYCTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GLYCTK (GLYCTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GLYCTK gRNA (BRDN0001145738)

Plasmid

#77908

Purpose

3rd generation lentiviral gRNA plasmid targeting human GLYCTK

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GLYCTK (GLYCTK Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK8 gRNA (BRDN0001148581)

Plasmid

#77885

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK8 gRNA (BRDN0001149279)

Plasmid

#77882

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AK8 gRNA (BRDN0001149503)

Plasmid

#77883

Purpose

3rd generation lentiviral gRNA plasmid targeting human AK8

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AK8 (AK8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge