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human p53-(310-360)

Plasmid

#24872

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains only the dimeric oligomerization domain …

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pMEH280 NLS dTomato 3x high affinity MS2 loops

Plasmid

#168211

Purpose

Mammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loops

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Pac

Plasmid

#126957

Purpose

Promoter acceptor vector. Minimal cargo vector; with eGFP without promoter in downstream secondary site. Allows introducing any part using BsmBI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSuper mouse Dicer3

Plasmid

#14778

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (Dicer1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pJYDN3p

Plasmid

#162457

Purpose

Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a1

Plasmid

#15745

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1 (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pBx1-VHH template3-3XMyc-Spacer

Plasmid

#176210

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template3-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-UBC:GFP

Plasmid

#68482

Purpose

Retrovirus expressing GFP driven by UBC promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR and UBC

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Plasmid

#192124

Purpose

pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry

Use

Lentiviral

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-CFP

Plasmid

#22221

Purpose

Lentiviral expression of Arch-CFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk2a2

Plasmid

#15744

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pLV CDHblast Rev-EGFP-V5

Plasmid

#175164

Purpose

Lentiviral expression of Rev-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC-riboB

Plasmid

#168975

Purpose

Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; riboB

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Expression in aspergillus nidu…

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::HygroR-bGHpA

Plasmid

#62436

Purpose

MXS_chaining vector with PGK::HygroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against hygromycin B with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk1a1

Plasmid

#15743

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
TOPO rtTA3-2A-Cre

Plasmid

#68448

Purpose

TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

rtTA3-2A-Cre

Use

Gmap

Expression

Bacterial

Mutation

Codon optimized for expression in mice

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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