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FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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G protein alpha-q-CFP

Plasmid

#66081

Purpose

This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

Depositor

Catherine Berlot

Publication

Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

Insert

G protein alpha-q-EE-CFP (Gnaq Mouse)

Tags

CFP was inserted internally into the alpha-q sequ…

Expression

Mammalian

Mutation

BamHI and SacI sites in alpha-q were removed with…

Promoter

CMV

Available since

July 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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R701-X65-527: His6-tev-Hs.CALM1(2-148)

Plasmid

#159693

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(2-148)

Depositor

Dominic Esposito

Publication

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(2-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP47

Plasmid

#101915

Purpose

Expresses SNAP47 in mammalian cells

Depositor

Thierry Galli

Publication

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP47 (SNAP47 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMCSF-R(mB)-luc

Plasmid

#12422

Depositor

Dong-Er Zhang

Publication

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

M-CSF receptor promoter fragment (CSF1R Human)

Use

Luciferase

Mutation

AML1 binding site mutation (created a KpnI site i…

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pEXL flag Smad3

Plasmid

#10920

Depositor

Bob Weinberg

Publication

Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

Insert

Smad3 (SMAD3 Human)

Tags

flag

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pSD080A

Plasmid

#218138

Purpose

Lentiviral vector expressing evolved degron SD40 fused to eGFP with mCherry control.

Depositor

David Liu

Publication

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40-eGFP-IRES2-mCherry

Use

Lentiviral

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pRSII402

Plasmid

#35434

Depositor

Steven Haase

Publication

Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

Type

Empty backbone

Use

Yeast integrating vector

Promoter

None

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Publication

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEN_TGmiRc3

Plasmid

#25749

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

GFP

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-L-R279E

Plasmid

#182282

Purpose

Mammalian expression of cAMPFIRE-L with R279E (non-cAMP-binding) mutation under a CMV promotor.

Depositor

Haining Zhong

Publication

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Publication

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Ha-Brca1 S1423A/S1524A

Plasmid

#32539

Depositor

Michael Kastan

Publication

Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Mutation

S1423A/S1524A

Promoter

CMV/rabbit B-Globin

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmAGO1

Plasmid

#22768

Depositor

Elisa Izaurralde

Publication

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

Argonaut-1, variant A (AGO1 Fly)

Use

Drosophila expression

Tags

EGFP

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1-L2/3A

Plasmid

#48

Depositor

Bruce Spiegelman

Publication

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

PGC-1a (Ppargc1a Mouse)

Tags

flag

Expression

Mammalian

Mutation

LXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pLS13-IL-10

Plasmid

#209128

Purpose

Transient mammalian expression of IL-10

Depositor

Tom de Greef

Publication

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

IL-10 (IL10 Human)

Expression

Mammalian

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBB

Plasmid

#53210

Purpose

Contains a 103bp telomeric track

Depositor

Jack Griffith

Publication

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TTAGGG(n) Track

Promoter

T7

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSicoR-mCh-GFPi

Plasmid

#21905

Depositor

Miguel Ramalho-Santos

Publication

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Insert

GFPi

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z13

Plasmid

#120056

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z05

Plasmid

#120048

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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