Addgene

Showing: 5261 - 5280 of 12549 results

Results per page:

Sort results by:

pET51b(+)_LAMA-F98 (for GFP)

Plasmid

#130714

Purpose

Plasmid encoding the bacterial expression of LAMA-F98 for GFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

LAMA-F98

Use

Tags

Hisx10

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Article

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-mCherry-WPRE

Plasmid

#127860

Purpose

pAAV plasmid expressing mCherry under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b LFn-Fla

Plasmid

#84871

Purpose

Bacterial expression of LFn-Fla fusion

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only
pME-memGFP-P2A

Plasmid

#80809

Purpose

Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

pME-memGFP-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSGEMKv10.1

Plasmid

#85704

Purpose

Xenopus laevis oocyte expression of Kv10.1

Depositor

Luis Pardo

Article

Jenke et al EMBO J. 2003 Feb 3;22(3):395-403.

Insert

KCNH1 (KCNH1 Human)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMV-VioB

Plasmid

#65348

Purpose

systhesis of vioB gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pKMV-VioD

Plasmid

#65350

Purpose

systhesis of vioD gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioD

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN0604 (pDEST, HygR)

Plasmid

#210483

Purpose

pDEST vector with HygR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDN0603 (pDEST, NatR)

Plasmid

#210482

Purpose

pDEST vector with NatR marker for gene expression in S. cerevisiae.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

Type

Empty backbone

Use

Synthetic Biology; Destination vector for gateway…

Tags

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RabV CVS-N2c(deltaG)-FlpO

Plasmid

#73473

Purpose

Expresses FlpO for circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26_MraYaa

Plasmid

#100166

Purpose

recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Chung et al Science. 2013 Aug 30;341(6149):1012-1016

Insert

mraY (phospho-MurNAc-pentapeptide translocase)

Use

Tags

MBP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-C-8His-Flag

Plasmid

#157678

Purpose

C-terminal tagging vector

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

8His-Flag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCoofy44

Plasmid

#55185

Purpose

Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

Expression

Bacterial

Mutation

Promoter

pPH (Polyhedrin promoter)

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK255

Plasmid

#171155

Purpose

Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Insert

I-SceI_sacB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-MCM4-N-22

Plasmid

#54168

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM4

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP-SUN1_785

Plasmid

#125852

Purpose

Expresses SUN1_785 tagged with GFP in mammalian cells

Depositor

Miki Hieda

Article

Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

Insert

SUN1_785 (SUN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
RIP-Timer (DM#285)

Plasmid

#15109

Depositor

Douglas Melton

Article

Dor et al Nature. 2004 May 6. 429(6987):41-6.

Insert

insulin II promoter (Ins2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge