We narrowed to 11,832 results for: NSI;
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Plasmid#240608PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on ACE2-clus…PromoterCMVAvailable SinceSept. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
CASTOR1-HA (D304A)
Plasmid#237419PurposeTransient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)DepositorAvailable SinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C(3+4)
Plasmid#240611PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on both ACE2…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACE2-Mutant-C(0+4)
Plasmid#240610PurposeTo test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsFLAG tagExpressionMammalianMutationArginine (R) and Lysine (K) residues on both ACE2…PromoterCMVAvailable SinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRK5R 15xGly
Plasmid#211355PurposeTransient expression of 15xGly-EGFP-3xHADepositorInsert15xGly-EGFP-3xHA
TagsEGFP-3xHAExpressionMammalianPromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTRNA scPhe-GAA
Plasmid#211359PurposeTransient expression of scPhe-GAADepositorInsertS. cerevisiae Phe-GAA tRNA
ExpressionMammalianAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-hGpr75
Plasmid#236746PurposeExpress 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-mGpr75
Plasmid#236744PurposeExpress 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG p85beta
Plasmid#237336Purposetransient overexpression in mammalian cellsDepositorAvailable SinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export
Plasmid#233382PurposeOptimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequenceDepositorInsertVEGFR1(ecto+TM)-ADAR2dd
TagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Myc-GATA3
Plasmid#232646Purposetransient expresses GATA3 in mammalian cellsDepositorAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m3
Plasmid#204465PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L125W-A127S mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m4
Plasmid#204466PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L125W-A127T mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m5
Plasmid#204467PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L125F-A127G-S129N mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m6
Plasmid#204468PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L125W-S129N-L130V mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m7
Plasmid#204469PurposeThis is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L125W-A127T-L128M mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMZ103-rbs1-m8
Plasmid#204470PurposeThis is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.DepositorInsertsUseSynthetic BiologyMutationLasR L128F-S129W mutationPromoterIn an operon and PLasAvailable SinceMarch 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ HA-BEX1
Plasmid#231002PurposeTransient overexpression of human BEX1 in mammalian cellsDepositorAvailable SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-AP4-cyto
Plasmid#229694PurposeTransient expression of EGFP AP4-cyto in mammalian cellsDepositorInsertEGFP; Control construct with tandem AP4 motifs (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-EGFP-FP4-cyto
Plasmid#229695PurposeTransient expression of FP4-cyto in mammalian cellsDepositorInsertEGFP, Mitochondira FP4 motifs from Listeria ActA (cytoplasm)
TagsEGFPExpressionMammalianPromoterCMV promoterAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GB4668
Plasmid#215272PurposeModule for the expression of the N. nambi HISPS gene under the ABA- responsive MAPKKK18 promoter and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertpMAPKKK18:HISPS.TNOS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pro::EX1-2(intΔNACs):GFP
Plasmid#218570PurposeTranscriptional reporter for ARF7 promoterDepositorInsertAT5G20730 (NPH4 Mustard Weed)
ExpressionPlantMutationMutation genomic sequence 85nt, 86nt from TA to C…Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMamCol1
Plasmid#215184PurposeAllows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coliDepositorInserttsPurple
UseAAV, CRISPR, and Synthetic Biology ; Recombinant …TagsSNAC-StrepII tagExpressionBacterial and MammalianPromoterTetON 3G Bidirectional for Mammalian and Trc with…Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48)-VSV
Plasmid#212742PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNUbo and VSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-VSV
Plasmid#212741PurposeConsitutive expression of NUbo-E3(48)-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-CUbo-VSV
Plasmid#212733PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-CUbo-VSV
Plasmid#212732PurposeConsitutive expression of CUbo-VSV in budding yeastDepositorInsertCUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-VSV
Plasmid#212729PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-VSV
Plasmid#212728PurposeConsitutive expression of NUbo-VSV in budding yeastDepositorInsertNUbo
UseIntegrative vectorTagsVSVExpressionYeastPromoterADH1Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-PTS1
Plasmid#209398PurposeTransiently expressing TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID
Plasmid#209397PurposeTransiently expressing TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-2
Plasmid#202188PurposeExpress 'Frost Lisbon' Lemon FLS2-2 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
TagsHAExpressionPlantPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:LLFLS2-1
Plasmid#202187PurposeExpress 'Frost Lisbon' Lemon FLS2-1 gene under the UBQ10 promoterDepositorInsertFlagellin sensing 2
TagsHAExpressionPlantPromoterAtUBQ10Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
TagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VC155
Plasmid#194046PurposeTransient expression of AtOEP7-VC155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
TagsAtOEP7 1–50aaExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-VN154
Plasmid#194045PurposeTransient expression of AtOEP7-VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Chloroplast outer envelope membrane)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
TagsAtOEP7 1–50aaExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB2890
Plasmid#193133PurposeA2 Proximal promoter sequence consisting of the target sequence for the gRNA1 (GB1838) at "d site" (-120 from TSS) and gRNA2 (GB1839) flanked by random sequences.DepositorInsertGB_SynP (A2) G1dG2b.1
UseSynthetic BiologyAvailable SinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
TagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only