We narrowed to 7,509 results for: PAC;

1. pET16-mHP1alpha

   Plasmid

   #181910

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMYC BRGI aa597-1407

   Plasmid

   #19146

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   human BRG1 aa 597-1407 (PCR product)

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

   Plasmid

   #157702

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pMMB67EH-dddAI-FLAG

   Plasmid

   #186560

   Purpose

   Broad host range regulated expression of the DddA immunity determinant with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

   Depositor

   Joseph Mougous

   Article

   Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

   Insert

   dddAI
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRGEN-CMV-VPR-WT CjCas9 V-WT

   Plasmid

   #169914

   Purpose

   Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   CjCas9
   

   Use

   CRISPR

   Tags

   2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TagBFP2-FKBP-INPP5ED477N

   Plasmid

   #202738

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   TagBFP2-FKBP-INPP5ED477N (INPP5E, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-mHP1alpha

   Plasmid

   #181911

   Purpose

   Bacterial protein expression vector for HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAD54_CAD29_H104A_pvp008

   Plasmid

   #124876

   Purpose

   Expresses hCAD His103Ala mutant in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Use

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   mutated His103 to Ala, deleted amino acids 1-3 an…

   Promoter

   T7

   Available since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-FRT-TO-FH-Nsp8-Nsp7_GC3opt

   Plasmid

   #157701

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp8-Nsp7_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

    Plasmid

    #197892

    Purpose

    Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    hM4D/2A/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMYC BRGI aa1400-1648

    Plasmid

    #19145

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    human BRG1 aa 1400-1648 (PCR product)

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid

    #140203

    Purpose

    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    crRNA array elcA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PgpdA

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid

    #157699

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pPSV39-dddAI-FLAG

    Plasmid

    #186558

    Purpose

    Broad host range regulated expression of the DddA with a C-terminal fusion to a FLAG epitope (DddAI-FLAG)

    Depositor

    Joseph Mougous

    Article

    Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

    Insert

    dddAI
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His6-SMT3-SopB(C460S)

    Plasmid

    #183677

    Purpose

    Recombinant Salmonella Typhimurium SopB (catalytically inactive)

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    His6-S. cerevisiae SMT3(2-101)

    Expression

    Bacterial

    Mutation

    C460S

    Promoter

    T7

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAD108_Arg273His_pCMV6Entry-hIRG1

    Plasmid

    #124878

    Purpose

    Expresses hCAD Arg273His mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Arg273 to His

    Promoter

    CMV

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. G30, Cdc20-mCherry-AID tagging

    Plasmid

    #130270

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid

    #157693

    Purpose

    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCAD96_hIRG1_4-461_pvp008

    Plasmid

    #124874

    Purpose

    Expresses hCAD Asn152Ser mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated Asn152 to Ser, deleted amino acids 1-3 an…

    Promoter

    T7

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-APLP1ICD-IRES-hrGFP

    Plasmid

    #107546

    Purpose

    AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

    Depositor

    Hélène Marie

    Article

    Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

    Insert

    APLP1ICD (APLP1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge