We narrowed to 22,280 results for: fus

1. pFX-mCherry-Rab5

   Plasmid

   #174455

   Purpose

   Express mCherry with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with early endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab5)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiEKAR2G1

   Plasmid

   #40177

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. AYBEv0.1

   Plasmid

   #193966

   Purpose

   A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

   Insert

   ecTadA8e-nSpCas9(D10A)-MPG(wt)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a, CBh

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCpf1-eBE-YE

   Plasmid

   #107689

   Purpose

   Expresses dLbCpf1-eBE-YE in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-eBE-YE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

   Promoter

   CMV

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-iRFP-Rab11

   Plasmid

   #174462

   Purpose

   Express iRFP with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   iRFP with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3xFLAG-CMV-14-Mfn1-T562A

   Plasmid

   #191976

   Purpose

   To produce mutants mimicking dephosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T562A (Mfn1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T562A

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG-CMV-14-Mfn1-T562D

   Plasmid

   #191977

   Purpose

   To produce mutants mimicking phosphorylated Mfn1

   Depositor

   Luca Scorrano

   Article

   Pyakurel et al Mol Cell. 2015 Apr 16;58(2):244-54. doi:

   Insert

   Mfn1 T562D (Mfn1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T562D

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti X2 Neo/pTER shEGFP#2 (w22-2)

   Plasmid

   #17480

   Purpose

   3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Enhanced green fluorescent protein shRNA #2
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221 Cas9-POLD3

   Plasmid

   #183197

   Purpose

   Entry clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   Cas9-POLD3
   

   Use

   CRISPR; Gateway entry clone

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFX-mito-MiCy

    Plasmid

    #174177

    Purpose

    Express MiCy with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    MiCy with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJR2956

    Plasmid

    #122533

    Purpose

    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBK-CMV-mUBP43

    Plasmid

    #12459

    Depositor

    Dong-Er Zhang

    Article

    Liu et al Mol Cell Biol. 1999 Apr . 19(4):3029-38.

    Insert

    UBP43 (Usp18 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pFX-β-tubulin-Venus

    Plasmid

    #175828

    Purpose

    Express Venus with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFX-Lifeact-mCherry

    Plasmid

    #175822

    Purpose

    Express mCherry with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with actin-targeting sequence
    

    Use

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMB

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-β-tubulin-iRFP

    Plasmid

    #175830

    Purpose

    Express iRFP with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    iRFP with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGAD-Atg17

    Plasmid

    #121386

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRXIP-Myc-SNAP29

    Plasmid

    #116944

    Purpose

    Expressing Myc-SNAP29 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    synaptosomal-associated protein, 29kDa (SNAP29 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-BD-cop1at(335-675)

    Plasmid

    #44973

    Depositor

    Thanos Halazonetis

    Article

    Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

    Insert

    BD-COP1 (COP1 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA-binding domain

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-334

    Promoter

    pCMV

    Available since

    June 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCph8

    Plasmid

    #22869

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2005 Nov 24. 438(7067):441-2.

    Insert

    pCph8
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    First 517 amino acids of Cph1 and the last 229 am…

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJR2955

    Plasmid

    #122550

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemNeptune2.5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-GLT1a-V5

    Plasmid

    #162517

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1a (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-GLT1b-V5

    Plasmid

    #162518

    Purpose

    Rat GLT-1a cDNA N-terminally tagged with EGFP with a v5 tag in the extracellular loop

    Depositor

    Josef Kittler

    Article

    Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

    Insert

    GLT1b (Slc1a2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221 Cas9-RFC5

    Plasmid

    #183200

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC5
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Use

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-28a-His6-SUMO-VAMP2

    Plasmid

    #135551

    Purpose

    Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP2
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFX-Venus-Sec61β

    Plasmid

    #174467

    Purpose

    Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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