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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,866 results for: cas9

Showing: 10561 - 10600 of 13866 results

  9. pEPOR1CB0023

    Plasmid
    #117538
    Purpose
    Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    sgRNA (Sp Cas9) - extended stem with U626 terminator
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    117538-plasmid-map-sequence-id-228054

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  16. pJK412

    Plasmid
    #155371
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    155371-plasmid-map-sequence-id-308999

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  17. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Mutation
    Promoter
    EFS, No promoter, and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  20. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  21. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153338-plasmid-map-sequence-id-306018

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  24. ePE6d

    Plasmid
    #229029
    Purpose
    Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
    Depositor
    Li Juan
    Article
    Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Feb. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    229029-plasmid-map-sequence-id-462729

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  35. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv November 07, 2024
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  39. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

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Showing: 10561 - 10600 of 13866 results