-
Plasmid#235803Purpose5' Operon Connector 3 in the M1 position used to construct operonsDepositorInsert5' Operon Connector 3
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_1-2_005_OC5'5
Plasmid#235805Purpose5' Operon Connector 5 in the M1 position used to construct operonsDepositorInsert5' Operon Connector 5
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_1-2_006_OC5'6
Plasmid#235806Purpose5' Operon Connector 6 in the M1 position used to construct operonsDepositorInsert5' Operon Connector 6
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_G_0_1-2_001_OC5'1
Plasmid#235801Purpose5' Operon Connector 1 in the M1 position used to construct operonsDepositorInsert5' Operon Connector 1
UseSynthetic BiologyTagsExpressionMutationnonePromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME_Cp_0_3b_029_mScarlet-I
Plasmid#235882PurposemScarlet-I N-tagDepositorInsertmScarlet-I N-tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-B/c
Plasmid#227963PurposeExpression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.DepositorInsertB/c
UseTagsExpressionPlantMutationPromoter35SAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJAK184.tetR.PT5-3.dCas9
Plasmid#232482Purposeoptimized ahTet inducible dCas9 for insertion into C. difficile genomeDepositorInsertCatalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
UseCRISPRTagsExpressionBacterialMutationPromoterTetR-PT5-3Available sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRTagsExpressionBacterialMutationPromoterP3 constitutive promoter and native promoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA
Plasmid#225079PurposeExpresses precursor peptide ChrA in E.coliDepositorInsertChrA
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pM-mKate2
Plasmid#228629PurposeMammalian expression of mKate2 and extra insert, for MuSIC barcode constructionDepositorInsertmKate2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pM-LSSmOrange
Plasmid#228619PurposeMammalian expression of LSSmOrange and extra insert, for MuSIC barcode constructionDepositorInsertLSSmOrange
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C66S
Plasmid#225081PurposeExpresses precursor peptide ChrA-C66S in E.coliDepositorInsertChrA-C66S
UseTagsHis6ExpressionBacterialMutationC66SPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mBeRFP
Plasmid#228620PurposeMammalian expression of mBeRFP and extra insert, for MuSIC barcode constructionDepositorInsertmBeRFP
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mTFP1
Plasmid#228621PurposeMammalian expression of mTFP1 and extra insert, for MuSIC barcode constructionDepositorInsertmTFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-CyOFP1
Plasmid#228623PurposeMammalian expression of CyOFP1 and extra insert, for MuSIC barcode constructionDepositorInsertCyOFP1
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mOrange2
Plasmid#228627PurposeMammalian expression of mOrange2 and extra insert, for MuSIC barcode constructionDepositorInsertmOrange2
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby3
Plasmid#228628PurposeMammalian expression of mRuby3 and extra insert, for MuSIC barcode constructionDepositorInsertmRuby3
UseTagsExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLei-wt-sfGFP
Plasmid#191113PurposeAn E. coli sfGFP wild type controlDepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1
Plasmid#228508PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I5CHO (#1)
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZB578_pB1H1_T7-RNAP_LARP-I
Plasmid#228507PurposeBacterial Hybrid Driver PlasmidDepositorInsertT7 RNAP, LARP-I
UseSynthetic BiologyTagsUmu Degron, GTG-start codonExpressionMutationR378K; S430P, N433T, S633P, F849I, F880Y; W727G, …PromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only