We narrowed to 32,111 results for: eng

1. p4B5-EGFP

   Plasmid

   #140324

   Purpose

   Detection of Dengue virus infection in live cells

   Depositor

   Alan Rothman

   Article

   Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

   Insert

   DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   2 conservative n.t. differences near 5' end …

   Promoter

   CMV

   Available Since

   May 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP91

   Plasmid

   #83548

   Purpose

   Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PGK1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PGK1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

   Plasmid

   #42337

   Purpose

   Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQAT22

   Plasmid

   #223394

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Flag HBx

   Plasmid

   #42596

   Depositor

   Ding Xue

   Article

   Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

   Insert

   Hepatitis B virus protein X
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQAT19

   Plasmid

   #223391

   Purpose

   T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FlagETR3 (CELF2)

   Plasmid

   #96900

   Purpose

   expression of Flag-tagged human CELF2 (aka CUGBP2)

   Depositor

   Thomas Cooper

   Article

   Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

   Insert

   human CELF2 cDNA (CELF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hAsCpf1(TYCV) (pY210)

   Plasmid

   #89351

   Purpose

   Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CMV

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV Puro SCRIB WT

   Plasmid

   #88886

   Purpose

   SCRIB WT in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Tags

   N/A

   Mutation

   N/A

   Promoter

   MSCV promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1 promoter construct

    Plasmid

    #20736

    Depositor

    Joshua Sanes

    Article

    Feng et al Neuron. 2000 Oct . 28(1):41-51.

    Insert

    Thy1 promoter (Thy1 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCG150_double_phiC31_attB

    Plasmid

    #169693

    Purpose

    cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

    Depositor

    Shih-Peng Chan

    Article

    Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. prDNA spacer-LacOx50-20xMS2-Hyg

    Plasmid

    #210219

    Purpose

    Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    50xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNICKclos1.0

    Plasmid

    #73639

    Purpose

    Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

    Depositor

    Sheng Yang

    Article

    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

    Inserts

    Cas9 nickase
    sGRNA to pyrE
    

    Use

    E.coli-clostridium shuttle vector

    Mutation

    D10A

    Promoter

    j23119 and ptb

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSin-NE1m

    Plasmid

    #172883

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons

    Depositor

    J. Julius Zhu

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Insert

    NE SENSOR
    

    Expression

    Mammalian

    Promoter

    sp6

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQAT21

    Plasmid

    #223393

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-FAS-GCaMP6f-WPRE

    Plasmid

    #216277

    Purpose

    AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

    Depositor

    Guohong Cui

    Article

    Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

    Insert

    GCaMP6f
    

    Use

    AAV and Cre/Lox

    Tags

    6XHIS and Xpress

    Promoter

    human synapsin 1 (hSyn)

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB1H1-Zif268

    Plasmid

    #12612

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Zif268 in pB1H1 (Egr1 Mouse)
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. plko-shmfn2-mito-GFP-ER tethering

    Plasmid

    #160509

    Purpose

    Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mito-GFP-ER tethering
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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