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HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

Plasmid

#217743

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRV1 (ADGRV1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5896

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

CELSR2 (CELSR2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG2 CTFdeltaTA-FLAG

Plasmid

#217733

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG2 (ADGRG2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-612

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

Plasmid

#69583

Purpose

Expresses p53 tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

Plasmid

#217707

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRL2 (ADGRL2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-824

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

Plasmid

#201217

Purpose

pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

Insert

ADGRL3 (Adgrl3 Mouse)

Tags

Flag and SNAP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

Plasmid

#209851

Purpose

Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

Depositor

Volker Busskamp

Article

Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

Insert

light-driven sodium pump ErNaR

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

Plasmid

#36881

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

GFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator

Tags

3 Myc tags

Expression

Mammalian

Mutation

deleted nucleotides after nucleotide 274, inserti…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mCerulean

Plasmid

#69581

Purpose

Expresses p53-L344P mutant tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

p53 L344P mutation that prevents oliogomerization…

Promoter

EF1alpha

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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human rSHIP2 (18-893)

Plasmid

#128568

Purpose

Expression of human SHIP2 in bacteria

Depositor

Christophe Erneux

Article

Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

Insert

SHIP2 (INPPL1 Human)

Tags

His Xpress (N-terminal)

Expression

Bacterial

Mutation

SHIP2 coding sequence from amino acid 18 (A) till…

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinHFDD

Plasmid

#134462

Purpose

M1 SpastinHFDD expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Expression

Mammalian

Mutation

Met to Ala at amino acid 87; His to Asp at amino …

Promoter

CMV

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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RalA (G23V)( full )-pcw107-V5

Plasmid

#64645

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

V5

Mutation

G23V

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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FUW-CreERT2-P2A-NeoR

Plasmid

#253966

Purpose

Lentiviral expression of tamoxifen-inducible CreERT2 with neomycin (G418) resistance (P2A-NeoR)

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

CreERT2

Use

Cre/Lox and Lentiviral

Promoter

UbC

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mPDZD8-3xFLAG (1-1147)

Plasmid

#253950

Purpose

Mammalian Expression of 3xFLAG-tagged mouse PDZD8

Depositor

Yusuke Hirabayashi

Article

Nakamura et al Nat Commun. 2025 Apr 17;16(1):3401. doi:

Insert

Pdzd8 (Pdzd8 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET_Y34X

Plasmid

#246084

Purpose

Stable expression of LYSET Y34X (isoform 2; Y72X in isoform 1)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET Y34X (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET

Plasmid

#246082

Purpose

Stable expression of LYSET (isoform 2)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET_R7W

Plasmid

#246083

Purpose

Stable expression of LYSET R7W (isoform 2; R45W in isoform 1)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET R7W (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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