We narrowed to 32,377 results for: FER

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pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-hPE(+)-Luc

Plasmid

#172003

Purpose

Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

Depositor

Daniel Herranz

Article

Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

Insert

Human PE sequence (hPE)

Use

Luciferase

Expression

Bacterial and Mammalian

Promoter

SV40 Promoter

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-TGMV-nCas9-EcKlenow (JO659)

Plasmid

#208951

Purpose

A variant CE1 construct with TGMV HUH enconuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

TGMV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-FBYNV-nCas9-EcKlenow (JO610)

Plasmid

#208950

Purpose

A variant CE1 construct with FBYNV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

FBYNV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-RepBM-nCas9-EcKlenow (JO606)

Plasmid

#208949

Purpose

A variant CE1 construct with RepBM HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

RepBm-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-TraI-nCas9-EcKlenow (JO604)

Plasmid

#208948

Purpose

A variant CE1 construct with TraI HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

TraI-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

Plasmid

#208945

Purpose

A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

Promoter

CMV and T7

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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iMb-Control-Mosaic (IR98.10)

Plasmid

#99748

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membrane

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-RECK-3'UTR-miR-21-mut

Plasmid

#53693

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Mutation

nucleotide sequence 1083-1086 is mutated to TTCG

Available Since

July 11, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-noSV40-humanEC1.45_p300peak1

Plasmid

#173947

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

p300 peak 1 from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Enhancer-ZC3H12D_PromoterMUT

Plasmid

#153068

Purpose

ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutagenesis to disrupt 3 BHLHE40 binding sites. N…

Promoter

None

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-nsp13 (SARS-CoV-2)

Plasmid

#169190

Purpose

Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

Insert

GST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)

Tags

GST

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-mouseEC1.45

Plasmid

#173972

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Mouse orthologous SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pMiR-RECK-3'UTR-miR-7-mut

Plasmid

#53692

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Mutation

nucleotide position 171-174 are mutated to GAAG

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO-Pgc-1alpha-EYFP

Plasmid

#241793

Purpose

Expression vector for mouse Pgc-1alpha with EYFP

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

EYFP

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV EfIa DIO CaBLAM

Plasmid

#244229

Purpose

Cre dependent bioluminescent reporter for calcium signaling

Depositor

Ute Hochgeschwender

Article

Lambert et al Nat Methods. 2025 Dec 2. doi: 10.1038/s4

Inserts

mNeonGreen
CaBLAM

Use

AAV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.10 EXOC3prom SV40

Plasmid

#246713

Purpose

To investigate the influence of a universal enhancer (SV40) on EXOC3 promoter activity (measured by luciferase expression)

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Inserts

EXOC3 Promoter (EXOC3 Human)
SV40 universal enhancer

Use

Luciferase

Mutation

WT

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4.10 promEXOC3

Plasmid

#205467

Purpose

Luciferase reporter of promoter activity

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

EXOC3 Promoter (EXOC3 Human)

Use

Luciferase

Promoter

EXOC3

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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