We narrowed to 16,363 results for: OMP;

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dFP-mini

Plasmid

#120543

Purpose

Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

Depositor

Brian Chow

Article

Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

Insert

de novo fluorescence-activating protein mini (dFP-mini)

Tags

6xHis - TEV cleavage

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLVE-FH-IN

Plasmid

#70071

Purpose

2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cells

Depositor

Thomas Tuschl

Article

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3/hArf5(WT)-EGFP

Plasmid

#79534

Purpose

Expresses C-terminally EGFP-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pOpLacI:mini35ss (GB0534)

Plasmid

#68179

Purpose

Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xOpLacI:mini35S

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBFP (GB0025)

Plasmid

#68193

Purpose

Provides the CDS of the Blue fluorescente protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

BFP coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pP35S (GB0030)

Plasmid

#68163

Purpose

Provides the CaMV 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pVG122

Plasmid

#211765

Purpose

Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

strongLOV

Use

For single-copy integration (if cut with pmei)

Tags

SV40 NLS and VP16

Expression

Yeast

Mutation

compared to EL222, strongLOV contains Glu84Asp mu…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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IDE-2Strep

Plasmid

#210355

Purpose

Expresses IDE fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

IDE (IDE Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mCherryEA

Plasmid

#207868

Purpose

Mitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mito-mCherryEA

Tags

4xCox8 mitochondrial targeting

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP delta607-623aa

Plasmid

#204544

Purpose

Expresses EGFP-tagged MVP delta607-623aa in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

delta607-623aa

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (WT)

Plasmid

#197008

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

-

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Nsp3 Mac1 - C2 (UCSF)

Plasmid

#169209

Purpose

Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

Depositor

James S Fraser

Article

Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

Insert

SARS-CoV-2 Nsp3 Mac1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Q-IL18-6

Plasmid

#166554

Purpose

scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide

Depositor

Susanne Gräslund

Insert

single chain-Fv, scFv

Use

Recombinant antibody expression

Tags

3xFLAG, Sortag, His6 and OmpA leader sequence

Expression

Bacterial

Promoter

LacZ

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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px330 RBP1 gRNA

Plasmid

#165593

Purpose

Insertion of RBP1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

RBP1 (Polr2a Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(G137I)

Plasmid

#133274

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G137I

Promoter

CMVd2

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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ETV3L_pet28a

Plasmid

#131666

Purpose

Bacterial expression of ETS gene ETV3L

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3L (ETV3L Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ELK1_pet28a

Plasmid

#131647

Purpose

Bacterial expression of ETS gene ELK1

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK1 (ELK1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-SAB-92018 vpr

Plasmid

#115822

Purpose

Encodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVSAB Vpr (HQ378594)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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