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pTMB352_ZFcharm_Kv1_split-inteins

Plasmid

#220848

Purpose

Lentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFS

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm Kv2

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214997

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (Tmem115 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

UBC

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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CXCR7-DuET

Plasmid

#213224

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CXCR7 (ACKR3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

Plasmid

#205160

Purpose

express β-ACTIN-3′UTR-8xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Tags

tagBFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mPD-L1-T2A-GFP

Plasmid

#205455

Purpose

lentiviral plasmid for expression of mouse PD-L1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mPD-L1 (Cd274 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-PUM1-rABE-Flag

Plasmid

#191385

Purpose

Doxycycline inducible expression of PUM1-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

PUM1-rABE (PUM1 Synthetic, Human)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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Arl6ip1-mEmerald

Plasmid

#186965

Purpose

Express Arl6ip1 with C-terminus mEmerald

Depositor

Ke Xu

Article

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

ARL6IP1 (ARL6IP1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_MicroID2-FLAG-NES

Plasmid

#188525

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a nuclear export sequence (NES) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHR Grb2-TagBFP

Plasmid

#188631

Purpose

Human Grb2 C-terminally labeled with TagBFP

Depositor

Jared Toettcher

Article

Farahani et al Elife. 2023 May 22;12:e82863. doi: 10.75

Insert

GRB2 (GRB2 Synthetic, Human)

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ND4.2-Right DdCBE-G1397-C- T1413I

Plasmid

#179685

Purpose

Expression of base editor in mammalian cells

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR

Expression

Mammalian

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-Nb127D01-mCherry

Plasmid

#171573

Purpose

Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-mCherry (CXCR2 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-E2F7-T2A-PuroR

Plasmid

#162348

Purpose

Lentiviral expression of E2F7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

E2F7 (E2F7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAG156 Mito-FAST

Plasmid

#130723

Purpose

Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

Depositor

Arnaud Gautier

Article

Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Mito-FAST

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_SZ1-RGG-GFP-RGG

Plasmid

#124932

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV14_NOP58

Plasmid

#73063

Purpose

expression clone for human NOP58 with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NOP58 ribonucleoprotein (NOP58 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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rTetR-NS3-VP64-p65_IRES_mCherry

Plasmid

#112628

Purpose

Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.

Depositor

John Ngo

Article

Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

Insert

rTetR-NS3-VP64-p65

Tags

AU1 (N term on NS3), HA (C term on NS3), and T7 (…

Expression

Mammalian

Mutation

NS3 mutant T54A

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEMS2241

Plasmid

#111896

Purpose

ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple320 (POU4F1 Human)

Use

AAV

Promoter

Ple320 (POU4F1 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSR02

Plasmid

#69149

Purpose

Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC41

Plasmid

#62332

Purpose

sgRNA (no RNA aptamer addition) with PCP-VP64 effector for mammalian cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA
PCP-VP64

Use

Lentiviral

Tags

VP64

Expression

Mammalian

Mutation

Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

Promoter

CMV and U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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