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Plasmid#196251PurposePlasmid for cloning the first CRISPR-Cas9 guide RNA of multiplex guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTL-Bag4-gRNA4
Plasmid#196255PurposePlasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAsDepositorInsertCRISPR-Cas9 guide RNA scaffold and multiple cloning site
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
tol2-GFAP::CD14
Plasmid#59593PurposeMagnetic cell sorting of ES cell-derived astrocytesDepositorInserthuman CD14 (CD14 Human)
UseTagsExpressionMammalianMutationPromotergfa2Available sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC_delta ald1
Plasmid#126708Purposevector for knocking out ald1 in Komagataella phaffiiDepositorInsertHIS4
UseTagsExpressionYeastMutationPromoterHisAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m4
Plasmid#84723PurposepVR2 but with G to U and A to C mutations following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at the first nucleotide after the…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m5
Plasmid#84724PurposepVR2 but with an insertion of 1 U immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationinsertion of 1 U between positions 39 and 40 of a…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m6
Plasmid#84725PurposepVR2 but with an insertion of 2 U's immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationinsertion of 2 Us between positions 39 and 40 of …PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m7
Plasmid#84726PurposepVR2 but with a G to U mutation immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutation at position 41 of annotated "…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2-m8
Plasmid#84727PurposepVR2 but with two G to U mutations immediately following the riboswitch aptamerDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutationG to U mutations at positions 40 and 41 of annota…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only