Addgene

We narrowed to 1,071 results for: HST

Showing: 781 - 810 of 1071 results

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pCMV5 Flag-Smad3 (146-425)

Plasmid

#14966

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 146-425

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad3 (220-425)

Plasmid

#14967

Depositor

Joan Massague

Article

Chen et al Cell. 2002 Jul 12. 110(1):19-32.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 220-425

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pRK Smad3LC Flag

Plasmid

#14829

Depositor

Rik Derynck

Article

Zhang et al Curr Biol. 1997 Apr 1. 7(4):270-6.

Insert

Smad3 LC (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

amino acids 146-425

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
9336-E27

Plasmid

#234310

Purpose

Gateway ORF Entry clone of human SMAD3 LSD-CSA with stop codon (for native or N-terminal fusions); Linker sites constitutively active and C-terminal sites dead

Depositor

Dominic Esposito

Insert

SMAD3 LSD-CSA (SMAD3 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

T179D/S204D/S208D/S213D/S422A/S423A/S425A; Linker…

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease fusion with non cleavable linker (N-terminal Twin-Strep-tag)

Plasmid

#228661

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Zika virus for biochemical assay

Depositor

Frank von Delft, ASAP Discovery

Insert

Zika virus portion of NS2B protease fused to NS3

Use

Tags

His-Twin Strep-Tev

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB-cT3G-cERP2-RAD51

Plasmid

#222567

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAD51

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAD51 (RAD51 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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LP376

Plasmid

#90200

Purpose

pCMV-myc with cDNA encoding HsTRAPPC10 aa residues 1000-1259

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC10 (TRAPPC10 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

aa residues 1000-1259

Promoter

CMV

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

Plasmid

#86874

Purpose

To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

MORF4L1 (MORF4L1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRK Smad3 deltaC Flag

Plasmid

#12626

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

Smad3 (SMAD3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

C-terminal truncated Smad3 (aa 1-382)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pRetroSuper-GFP Smad3

Plasmid

#15723

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad3 shRNA (SMAD3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
Rc/CMV cyclin B2

Plasmid

#10911

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin B2 (CCNB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

T178V, S203A, S207A, S212A

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pRetroSuper-shYes

Plasmid

#26987

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

shRNA Yes (YES1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 LC

Plasmid

#12633

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 LC (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LC segment of Smad3 (aa 146-425)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 C

Plasmid

#12634

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 C (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C segment of Smad3 (aa 199-425)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 NL

Plasmid

#12632

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 NL (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

N-L segment of Smad3 (aa 1-211)

Promoter

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
XFIVP Smad3

Plasmid

#14826

Depositor

Rik Derynck

Article

Feng et al Genes Dev. 1998 Jul 15. 12(14):2153-63.

Insert

Smad3 (SMAD3 Human)

Use

Tags

VP16 activation domain

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
GST-Smad3-MH1

Plasmid

#16493

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-200 of Smad3

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pHAGE-EF1a-HSF1-GFP

Plasmid

#227820

Purpose

Expresses HSF1 fused to GFP from EF1a promoter

Depositor

Jeffrey Gerst

Article

Pataki et al bioRxiv 2024.10.05.616786

Insert

HSF1 (HSF1 Human)

Use

Lentiviral

Tags

3xHA and GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC10_pPromALB_STARRseq

Plasmid

#220289

Purpose

STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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dGFP-Rab27a

Plasmid

#170862

Purpose

Mammalian expression of dGFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Use

Tags

dEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(K133R)

Plasmid

#125571

Purpose

Expresses the hRad51(K133R) protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

K133R

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smad3

Plasmid

#11742

Depositor

Jeff Wrana

Article

Labbe et al Mol Cell. 1998 Jul . 2(1):109-20.

Insert

SMAD3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5M-ADAM10

Plasmid

#31717

Depositor

Rik Derynck

Article

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

ADAM10 (ADAM10 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GFP-Rab27a

Plasmid

#170861

Purpose

Mammalian expression of His-GFP-Rab27a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-27a (RAB27A Human)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LP377

Plasmid

#90201

Purpose

pCMV-myc with cDNA encoding HsTRAPPC11 aa residues 701-1133

Depositor

Lotte Pedersen

Article

Schou et al Cilia. 2014 Jun 18;3:6. doi: 10.1186/204

Insert

TRAPPC11 (TRAPPC11 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

aa residues 701-1133

Promoter

CMV

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIH-Flag Smad3 WT

Plasmid

#27025

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only
pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only