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Plasmid#236489PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Hyg.DepositorInsertmCherry
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Nat
Plasmid#236476PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-Hyg
Plasmid#236495PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Hyg.DepositorInsertDendra2
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Hyg
Plasmid#236483PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-Hyg
Plasmid#236492PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-Hyg
Plasmid#236477PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-Nat
Plasmid#236479PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.DepositorInsertsfGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-Nat
Plasmid#236482PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0-Vamp2-TagIN-HA
Plasmid#196494PurposeTo tag endogenous mouse VAMP2 with HADepositorInsertsHA
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
KBB11
Plasmid#185422PurposeFull-length KAP60/SRP1DepositorInsertKAP60
UseTagsExpressionYeastMutationPromoterendogenousAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3Q->E
Plasmid#110538PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationQ to E mutations in Nab3 low complexity domainPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK500
Plasmid#72245PurposeProduces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp20>Phe mutant (EcPurE1-W20F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to Arg and cha…PromoterT7 and endogenousAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion mAID-mCherry with Neo
Plasmid#113831PurposeEasyFusion vector for targeting endogeneous genes with mAID-mCherryDepositorInsertmAID-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CelTag Plasmid
Plasmid#66562PurposeEncodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.DepositorInsertCelTag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCM-HA-Gas1
Plasmid#38313DepositorInsertGas1 (GAS1 Budding Yeast)
UseTags2xHAExpressionMutationcontains promoter regionPromoterEndogenousAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-LEXY (pDN157)
Plasmid#72658PurposeConstitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue lightDepositorInsertH2B-GFP-LEXY
UseTagsExpressionMutationPromoterCMVAvailable sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-halo-HA-p2a-neoR
Plasmid#161977Purposehomologous donor template for endogenous taggingDepositorInsertGAlinker-HA-halo-HA-p2a-neoR
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-puroR-p2a-HA-halo
Plasmid#161979Purposehomologous donor template for endogenous taggingDepositorInsertpuroR-p2a-HA-halo-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mNeonGreen-G418
Plasmid#236484PurposePlasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mStayGold-G418
Plasmid#236487PurposePlasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on G418.DepositorInsertmStayGold
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mCherry-G418
Plasmid#236490PurposePlasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on G418.DepositorInsertmCherry
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-sfGFP-G418
Plasmid#236481PurposePlasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.DepositorInsertsfGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-Dendra2-G418
Plasmid#236496PurposePlasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.DepositorInsertDendra2
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-mScarlet-G418
Plasmid#236493PurposePlasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.DepositorInsertmScarlet
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAPInt-GFP-G418
Plasmid#236478PurposePlasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.DepositorInsertGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEARBOCS-v0- Gfap-TagIN-mCherry
Plasmid#196490PurposeTo tag endogenous mouse GFAP with mCherryDepositorInsertsmCherry
gRNA
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB ID1-iCreERT2
Plasmid#139280PurposeHuman ID1-iCreERT2 transgene piggyBac transposonDepositorInsertID1 (ID1 Human)
UseCre/LoxTagsExpressionMammalianMutationReplaced the ID1 protein coding sequence in the t…PromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-blastR-p2a-v5-dL5
Plasmid#161980Purposehomologous donor template for endogenous taggingDepositorInsertblastR-p2a-v5-dL5-GSlinker
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-v5-dL5-p2a-blastR
Plasmid#161981Purposehomologous donor template for endogenous taggingDepositorInsertGSlinker-V5-dL5-P2A-blastR
UseTagsExpressionMutationPromoterAvailable sinceJan. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYCipi
Plasmid#53279PurposeContains crtE, crtB, crtI, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and thereby produces lycopene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_Donor (EMM0021)
Plasmid#63934Purpose3X Flag with P2A-Neo for 3' tagging of endogenous proteinsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsGlycine-Serine-3XFlag-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-mNG
Plasmid#179817PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-puro-3Myc:mNG:3Myc
Plasmid#179803PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3Myc
Plasmid#221046PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xMyc epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-CLIPtag
Plasmid#179787PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertCLIP
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZnF-hSyn-DIO-GPHNFingR-eGFP
Plasmid#200279PurposeGenetically encoded fluorescent probe for labelling endogenous inhibitory synapses in vivoDepositorArticleInsertzFn-FingR-GPHN-eGFP
UseAAVTagseGFPExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPLV_C1
Plasmid#186415PurposeEndogenous murine RNase inhibitor production for cell-free lysateDepositorInsertMurine RNase Inhibitor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ZnF-hSyn-DIO-PSD95FingR-mGreenLantern
Plasmid#200278PurposeGenetically encoded fluorescent probe for labelling endogenous excitatory synapses in vivoDepositorArticleInsertZnF-FingR-PSD95-mGreenLantern
UseAAVTagsmGreenLanternExpressionMammalianMutationPromoterhuman SynapsinAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dazl-CPG-TV
Plasmid#70144Purposea targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyADepositorInsert5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
UseMouse Targeting and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRc/CMV HA TrkB
Plasmid#39978DepositorInsertTrkB (Ntrk2 Rat)
UseTagsBM40 signal sequence and HAExpressionMammalianMutationendogenous signal sequence was replaced with BM40…PromoterCMVAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a
Plasmid#183567PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)DepositorInsertUnattenuated
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7.1-phleo-mNG
Plasmid#181935PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-blast-His:mNG:His
Plasmid#201094PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-3FLAG
Plasmid#221045PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsert3xFLAG epitopes
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6-puro-3Ty:tagRFPt:3Ty
Plasmid#201089PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInserttagRFPt
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv6.1-phleo-Ty:mNG:Ty
Plasmid#181936PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmNG
UseUnspecifiedTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only