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TMED3-hLOV-TEVcs-GAL4bd-VP64-V5

Plasmid

#170996

Purpose

HiLITR transcription factor with full-length TMED3 targeting sequence (ER/Golgi)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

TMED3(FL)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (TMED3 Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pMBS-869

Plasmid

#218021

Purpose

ER retention signal (DGDL) for protein targeting to ER

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

ER retention signal (DGDL)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-eGFP-KDEL

Plasmid

#177729

Purpose

ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Use

Tags

KDEL, Myc, and eGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA2

Plasmid

#171000

Purpose

HiLITR protease with MAVS [R537A, R538A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[R537A, R538A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[R537A, R538A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS-mTA5

Plasmid

#171003

Purpose

HiLITR protease with MAVS [Y536delinsFIVLI, R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

Depositor

Alice Ting

Article

Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A]

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

MAVS(tmd)[Y536delinsFIVLI, R537A]

Promoter

pTRE-tight (TetOn)

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut4

Plasmid

#18087

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut4 (SLC2A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut2

Plasmid

#18086

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut2 (SLC2A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pJW1665

Plasmid

#112039

Purpose

Integrating plasmid for expression of ER localized mCherry at TRP1 locus

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ER-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

TPI

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTLNX

Plasmid

#49032

Purpose

FX cloning Xenopus oocyte expression vector with SP6 promoter

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Xenopus oocytes expression

Tags

Expression

Mutation

Promoter

SP6

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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FRP1625

Plasmid

#64537

Purpose

Expresses LexA-ER-B42 in yeast cells

Depositor

Joerg Stelling

Article

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Insert

LexA-ER-B42

Use

Tags

Expression

Yeast

Mutation

Promoter

ACT1

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-CLIPf

Plasmid

#200787

Purpose

For the mammalian expression of CLIPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

CLIPf-HisTag-FLAG

Use

Tags

CLIPf

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SNAPf

Plasmid

#200788

Purpose

For the mammalian expression of SNAPf-HisTag-FLAG fusion protein.

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

SNAPf-HisTag-FLAG

Use

Tags

SNAPf

Expression

Mammalian

Mutation

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCVD315

Plasmid

#63737

Purpose

Derivative of pKO-1 carrying the multiple cloning region from M13mp19RF

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

PLIN3 (PLIN3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pERB264

Plasmid

#67764

Purpose

Tags peroxisomes with GFP and Halotag

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

PEX3-GFP-Halo

Use

Tags

GFP and Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCVD364

Plasmid

#63739

Purpose

V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Insert

nanH

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pJW1511

Plasmid

#62365

Purpose

pEF1a-puro-T2A-ATHmmuRPL10A

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

Puro-T2A-AVITEVHAmmuRPL10A

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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p7XNH3

Plasmid

#47064

Purpose

FX cloning E.coli expression vector with T7 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pERB254

Plasmid

#67762

Purpose

Tags mitochondria with Halotag and GFP

Depositor

Michael Lampson

Article

Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

Insert

Halo-GFP-Mito

Use

Tags

GFP and Halotag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pER-rxRFP1.4

Plasmid

#67842

Purpose

redox-sensitive red fluorescent protein rxRFP1.4, endoplasmic reticulum localized

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.4

Use

Tags

ER localization, ER retention, and His Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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