We narrowed to 2,098 results for: Pam

1. MSP1594

   Plasmid

   #65775

   Purpose

   Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGK112

   Plasmid

   #228127

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpCas9-P2A-puro

   Plasmid

   #214358

   Purpose

   Expresses SpCas9 and puromycin resistance genes

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 and puromycin resistance genes
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGK111

   Plasmid

   #228126

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SchBE4max

   Plasmid

   #192135

   Purpose

   Expresses AncBE4max and nSchCas9

   Depositor

   Yongming Wang

   Article

   Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

   Insert

   AncBE4max, nSchCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SchCas9 (D9A)

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCALNL-eGFP-NT1-6-gix-6-NT1

   Plasmid

   #81204

   Purpose

   contains two target sites consisting of PAM-NT1-6bp-gix psuedo site-6bp-NT1-PAM flanking a kan/pA as previously described in the pCALNL-eGFP plasmid

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   gix-Neo-gix deletion cassette upstream of EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCBa

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-443_Cacnes_HisB

   Plasmid

   #225608

   Purpose

   Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. MP6-SlugABEN-738

    Plasmid

    #214348

    Purpose

    Expresses N-terminus of ABE, and untargeted Bacterial Mutagenesis

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    dnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SpCas9HF1-P2A-puro

    Plasmid

    #214357

    Purpose

    Expresses SpCas9HF1 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9HF1 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-SlugCas9-P2A-puro

    Plasmid

    #214353

    Purpose

    Expresses SlugCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEND-349_Cacnes_ori

    Plasmid

    #225604

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEND-320_Cacnes_ori

    Plasmid

    #225602

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEND-321_Cacnes_ori

    Plasmid

    #225603

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a high-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEND-350_Cacnes_ori

    Plasmid

    #225605

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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