We narrowed to 1,606 results for: Sp1

1. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

   Plasmid

   #193853

   Purpose

   Promoterless insertion vector for split HygR/mScarlet-I landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' rps-0::HygR + 5' mScarlet-I
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-0p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

   Plasmid

   #169182

   Purpose

   Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

   Plasmid

   #205256

   Purpose

   S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP in pDBEcR-pIND(SP1)/Bla

   Plasmid

   #201557

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing DBEcR (3xmyc- tagged) and FLAG- EGFP; blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIMD(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

   Plasmid

   #201560

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP in pDBEcR-pIND(SP1)-DD/Bla

   Plasmid

   #201559

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP in pDBEcR-pIND(SP1)-W/Bla

   Plasmid

   #201558

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc and FLAG-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 SARS-CoV2 TEV-NSP16

   Plasmid

   #154414

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR223 SARS-CoV2 TEV-NSP14

   Plasmid

   #154412

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223 SARS-CoV2 TEV-NSP12

    Plasmid

    #154410

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX nsp1 K164A/H165A CoV2

    Plasmid

    #175514

    Purpose

    For protein expression of K164A/H165A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    K164A/H165A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX nsp1 R124A/K125A CoV2

    Plasmid

    #175513

    Purpose

    For protein expression of R124A/K125A nsp1 CoV2

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    R124A/K125A nsp1 CoV2
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223 SARS-CoV2 TEV-NSP1

    Plasmid

    #154400

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pMJ273: pRS426-His-MBP-GtFz1_PSP1

    Plasmid

    #205258

    Purpose

    S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMJ127: pRS426-His-MBP-NlovFz2_PSP1

    Plasmid

    #205257

    Purpose

    S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    NlovFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223 SARS-CoV2 TEV-NSP13

    Plasmid

    #154411

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    July 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. -2033/+750 hTSP1 promoter CAT

    Plasmid

    #15815

    Depositor

    Paul Bornstein

    Insert

    TSP1 promoter (THBS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pRK-flag-USP19 494-1318

    Plasmid

    #78587

    Purpose

    Expression flag-USP19 494-1318 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 494-1318 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    deleted amino acid 1-493

    Promoter

    CMV

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only